COMPARISON OF PARTIAL DENATURATION MAPS WITH THE KNOWN SEQUENCE OF SIMIAN VIRUS-40 AND PHI-X174 REPLICATIVE FORM DNA

被引：35

作者：

FUNNELL, BE ^{[1
]}

INMAN, RB ^{[1
]}

机构：

[1] UNIV WISCONSIN,DEPT BIOCHEM,MADISON,WI 53706

来源：

JOURNAL OF MOLECULAR BIOLOGY | 1979年 / 131卷 / 02期

关键词：

D O I：

10.1016/0022-2836(79)90079-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Electron microscope partial denaturation maps of two viral DNAs, simian virus 40 and φX174 replicative form, have been obtained. A simple computer program has been developed to predict denaturation maps from any given DNA sequence, based on the percentage of A · T base-pairs along the molecule. Maps constructed from the SV40 DNA and φX174 replicative form DNA base sequence show a good correlation with the experimental maps. The results show that the regions of a DNA molecule that denature first are, in fact, those regions with the highest content of adenine and thymine base-pairs. © 1979.

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页码：331 / 340

页数：10

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