INHIBITION OF SV40 DNA-REPLICATION INVITRO BY CHLORPROMAZINE

被引：0

作者：

HIRAI, H ^{[1
]}

TAKEDA, S ^{[1
]}

NATORI, S ^{[1
]}

SEKIMIZU, K ^{[1
]}

机构：

[1] UNIV TOKYO, FAC PHARMACEUT SCI, BUNKYO KU, TOKYO 113, JAPAN

来源：

BIOLOGICAL & PHARMACEUTICAL BULLETIN | 1993年 / 16卷 / 06期

关键词：

SV40; REPLICATION; CHLORPROMAZINE; PHOSPHOLIPID;

D O I：

暂无

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

We examined the inhibitory effect of chlorpromazine, a phenothiazine derivative, on SV40 DNA replication in a HeLa cell extract. The inhibition by chlorpromazine was reversed by cardiolipin, phosphatidylinositol, phosphatidylglycerol, and phosphatidylserine. Studies on a staged reaction showed that the pre-elongation step was sensitive to chlorpromazine: chlorpromazine-resistant DNA synthesis was observed when the drug was added after the pre-elongation step. Analysis of replication products by agarose gel electrophoresis revealed that some steps of DNA chain elongation and maturation of closed circular forms were also sensitive to this drug.

引用

页码：565 / 567

页数：3

共 25 条

[1] EFFECT OF AY-9944 AND CHLORPROMAZINE ON CONCANAVALIN A-INDUCED STIMULATION OF HUMAN-LYMPHOCYTES [J].

BARBU, V ;

MAZIERE, JC ;

MAINDRAULT, F ;

MAZIERE, C ;

RAMPINI, C ;

ROUX, C ;

POLONOVSKI, J .

BIOCHEMICAL PHARMACOLOGY, 1987, 36 (03) :353-356

[2]

BOHR V, 1986, CANCER RES, V46, P2929

[3]

CHALLBERG MD, 1989, ANNU REV BIOCHEM, V58, P671

[4] FURTHER-STUDIES ON F1-ATPASE INHIBITION BY LOCAL-ANESTHETICS [J].

CHAZOTTE, B ;

VANDERKOOI, G ;

CHIGNELL, D .

BIOCHIMICA ET BIOPHYSICA ACTA, 1982, 680 (03) :310-316

[5] CHLORPROMAZINE - A POTENTIAL ANTICANCER AGENT [J].

DARKIN, S ;

MCQUILLAN, J ;

RALPH, RK .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1984, 125 (01) :184-191

[6] IRREVERSIBLE BINDING OF THE CHLORPROMAZINE RADICAL CATION AND OF PHOTOACTIVATED CHLORPROMAZINE TO BIOLOGICAL MACROMOLECULES [J].

DEMOL, NJ ;

BUSKER, RW .

CHEMICO-BIOLOGICAL INTERACTIONS, 1984, 52 (01) :79-92

[7] REACTION OF LOCAL ANESTHETICS WITH PHOSPHOLIPIDS - POSSIBLE CHEMICAL BASIS FOR ANESTHESIA [J].

FEINSTEIN, MB .

JOURNAL OF GENERAL PHYSIOLOGY, 1964, 48 (02) :357-&

[8] BINDING OF SIMIAN-VIRUS 40 LARGE TUMOR-ANTIGEN TO PHOSPHOLIPID-VESICLES [J].

HIRAI, H ;

TAKAHASHI, N ;

NATORI, S ;

SEKIMIZU, K .

BIOCHIMICA ET BIOPHYSICA ACTA, 1991, 1090 (03) :305-310

[9] INHIBITORY-ACTION OF PHOSPHOLIPID-INTERACTING DRUGS ON TRANSCRIPTION INITIATION IN A NUCLEAR EXTRACT OF EHRLICH ASCITES TUMOR-CELLS [J].

HIRAI, H ;

NATORI, S ;

SEKIMIZU, K .

BIOCHIMICA ET BIOPHYSICA ACTA, 1991, 1088 (02) :191-196

[10]

HURWITZ J, 1990, J BIOL CHEM, V265, P18043

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