Chemical constituents from endophytic fungus Plectosphaerella cucumerina YCTA2Z1 of Cynanchum auriculatum

Objective: To study the chemical constituents from the EtOAc extract of endophytic fungal Plectosphaerella cucumerina YCTA2Z1. Methods: The chemical constituents were isolated and purified by silica gel column, Sephadex LH-20, and reverse-phase C-18 column chromatography as well as crystallization. Results: Thirteen compounds were isolated from the EtOAc extract of the fungal strain YCTA2Z1. Their chemical structures were elucidated according to the spectral evidence. They were identified as caudatin (1), baishouwubenzophenone (2), cynandione B (3), asterbatanoside A (4), p-hydroxyphenethyl-O-beta-D-glycoside (5), caffeic acid (6), ferulic acid (7), 2,5-dihydroxyacetophenone (8), protocatechuic acid (9), vanillic acid (10), stearic acid (11), azelaic acid (12), and succinic acid (13). Conclusions: It is the first chemical study on endophytic fungi from Cynanchum auriculatum and all the compounds are obtained from the species, the genus, as well as the family Plectosphaerellaceae for the first time. (c) 2018 Tianjin Press of Chinese Herbal Medicines. Published by Elsevier B.V. All rights reserved.