ISOLATION OF GLN- MUTANTS THROUGH TRANSPOSON, TN-917, MUTAGENESIS IN BACILLUS-BREVIS

被引：0

作者：

MISHRA, HS ^{[1
]}

MISHRA, DP ^{[1
]}

GARG, GK ^{[1
]}

机构：

[1] GOVIND BALLABH PANT UNIV AGR & TECHNOL,DEPT BIOCHEM,PANTNAGAR 263145,UTTAR PRADESH,INDIA

来源：

INDIAN JOURNAL OF BIOCHEMISTRY & BIOPHYSICS | 1991年 / 28卷 / 5-6期

关键词：

D O I：

暂无

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Transposon, Tn917, carried on pTV1 plasmid has been used successfully to mutagenise Bacillus brevis. The transposon showed preference for insertion at an "aro" site. A second insertional event after elimination of the preferred site with ethidium bromide/acridine orange treatment has permitted isolation of Gln- mutants in B. brevis.

引用

页码：472 / 475

页数：4