PROCEDURE FOR ISOLATION OF SPONTANEOUS MUTANTS WITH TEMPERATURE SENSITIVE SYNTHESIS OF RNA AND-OR PROTEIN

被引：48

作者：

ISAKSSON, LA ^{[1
]}

SKOLD, SE ^{[1
]}

SKJOLDEBRAND, J ^{[1
]}

TAKATA, R ^{[1
]}

机构：

[1] UNIV UPPSALA, DEPT MOLEC BIOL, WALLENBERG LAB, S-75237 UPPSALA, SWEDEN

来源：

MOLECULAR AND GENERAL GENETICS | 1977年 / 156卷 / 03期

关键词：

D O I：

10.1007/BF00267177

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A procedure for the isolation of spontaneous temperature sensitive mutants of Escherichia coli was developed. They are selected as survivors at high temperature against the combined killing effects exerted by a temperature inducible .lambda. prophage and either streptomycin plus ampillicin or ampicillin plus cycloserine. The mutants so obtained are blocked in vivo in the synthesis of RNA and/or protein or at restrictive temperature.

引用

页码：233 / 237

页数：5

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