DIRECT ENANTIOMERIC SEPARATION OF CIS-(+/-)DILTIAZEM IN PLASMA BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH OVOMUCOID COLUMN

被引：5
作者：
ROSELL, G [1 ]
CAMACHO, A [1 ]
PARRA, P [1 ]
机构：
[1] UNIV AUTONOMA BARCELONA,HOSP ST PAU,DEPT PHARMACOL & PSYCHIAT,E-08025 BARCELONA,SPAIN
来源：
JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1993年 / 619卷 / 01期
关键词：
D O I：
10.1016/0378-4347(93)80449-E
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A method is proposed for the direct enantiomeric separation of cis-(+/-)diltiazem in plasma. The method is based on a commercial Ultron ES-OVM column with a mobile phase of different mixtures of ethanol-phosphate buffer. The degree of extraction of the enantiomers was more than 86%. The detection limit of the method used was 5 ng of racemic mixture on column (coefficient of variation = 13%), corresponding to 3.2 ng for cis-(+)diltiazem and 1.8 ng for cis-(-)diltiazem.
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页码：87 / 92
页数：6
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