LARGE-SCALE SYNTHESIS OF TRIPLE-HELIX FORMING OLIGONUCLEOTIDES USING A CONTROLLED-PORE GLASS SUPPORT

被引：0

作者：

MURPHY, M ^{[1
]}

RIEGER, M ^{[1
]}

JAYARAMAN, K ^{[1
]}

机构：

[1] TRIPLEX PHARMACEUT,9391 GROGANS MILL RD,THE WOODLANDS,TX 77380

来源：

BIOTECHNIQUES | 1993年 / 15卷 / 06期

关键词：

D O I：

暂无

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Triple helix forming oligonucleotides that involve purine:purine:pyrimidine interactions are purine-rich oligonucleotides containing 60%-90% G and are usually 25-40 bases long. Synthesis and purification of G-rich oligonucleotides of this length can be difficult even at small scales. Procedures to synthesize these compounds at 200 to 400 mumol scales with high coupling efficiency have been developed using a controlled-pore glass support.

引用

页码：1004 / &

共 23 条

[1] LARGE-SCALE SYNTHESIS OF OLIGODEOXYRIBONUCLEOTIDE PHOSPHOROTHIOATE USING CONTROLLED-PORE GLASS AS SUPPORT [J].

RAVIKUMAR, VT ;

ANDRADE, M ;

WYRZYKIEWICZ, TK ;

SCOZZARI, A ;

COLE, DL .

NUCLEOSIDES & NUCLEOTIDES, 1995, 14 (06) :1219-1226

[2] SYNTHESIS OF TRIPLE-HELIX FORMING OLIGONUCLEOTIDES WITH A STRETCHED PHOSPHODIESTER BACKBONE [J].

RAO, TS ;

JAYARAMAN, K ;

DURLAND, RH ;

REVANKAR, GR .

NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS, 1994, 13 (1-3) :255-273

[3] SYNTHESIS OF TRIPLE-HELIX FORMING OLIGONUCLEOTIDES WITH A STRETCHED PHOSPHODIESTER BACKBONE [J].

RAO, TS ;

JAYARAMAN, K ;

REVANKAR, GR .

TETRAHEDRON LETTERS, 1993, 34 (39) :6189-6192

[4] SYNTHESIS OF TRIPLE-HELIX FORMING OLIGONUCLEOTIDES CONTAINING 2'-DEOXYFORMYCIN-A [J].

RAO, TS ;

HOGAN, ME ;

REVANKAR, GR .

NUCLEOSIDES & NUCLEOTIDES, 1994, 13 (1-3) :95-107

[5] SYNTHESIS AND PROPERTIES OF CHOLESTERYL-MODIFIED TRIPLE-HELIX FORMING OLIGONUCLEOTIDES CONTAINING A TRIGLYCYL LINKER [J].

VU, H ;

HILL, TS ;

JAYARAMAN, K .

BIOCONJUGATE CHEMISTRY, 1994, 5 (06) :666-668

[6] SYNTHESIS OF CHOLESTERYL SUPPORTS AND PHOSPHORAMIDITE FOR AUTOMATED DNA-SYNTHESIS OF TRIPLE-HELIX FORMING OLIGONUCLEOTIDES (TFOS) [J].

VU, H ;

SINGH, P ;

LEWIS, L ;

ZENDEGUI, JG ;

JAYARAMAN, K .

NUCLEOSIDES & NUCLEOTIDES, 1993, 12 (08) :853-864

[7] SYNTHESIS, PURIFICATION AND PHYSICAL-PROPERTIES OF CHOLESTERYL AND PROPYLAMINE MODIFIED TRIPLE-HELIX FORMING OLIGONUCLEOTIDES [J].

VU, H ;

JOYCE, N ;

MURPHY, M ;

JAYARAMAN, K ;

SPIESS, E .

ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 1994, 207 :60-CARB

[8] LARGE-SCALE ISOLATION OF EXPRESSION VECTOR CASSETTE BY MAGNETIC TRIPLE-HELIX AFFINITY CAPTURE [J].

SONTI, SV ;

GRIFFOR, MC ;

SANO, T ;

NARAYANSWAMI, S ;

BOSE, A ;

CANTOR, CR ;

KAUSCH, AP .

NUCLEIC ACIDS RESEARCH, 1995, 23 (19) :3995-3996

[9] Significant improvement of quality for long oligonucleotides by using controlled pore glass with large pores [J].

Kozlov, IA ;

Dang, M ;

Sikes, K ;

Kotseroglou, T ;

Barker, DL ;

Zhao, CF .

NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS, 2005, 24 (5-7) :1037-1041

[10] LARGE-SCALE SYNTHESIS OF OLIGONUCLEOTIDES VIA PHOSPHORAMIDITE NUCLEOSIDES AND A HIGH-LOADED POLYSTYRENE SUPPORT [J].

WRIGHT, P ;

LLOYD, D ;

RAPP, W ;

ANDRUS, A .

TETRAHEDRON LETTERS, 1993, 34 (21) :3373-3376

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