BARIUM AND CALCIUM STIMULATE SECRETION FROM DIGITONIN-PERMEABILIZED BOVINE ADRENAL CHROMAFFIN CELLS BY SIMILAR PATHWAYS

被引:33
作者
TERBUSH, DR [1 ]
HOLZ, RW [1 ]
机构
[1] UNIV MICHIGAN,DEPT PHARMACOL,ANN ARBOR,MI 48109
关键词
ATP; BARIUM; CALCIUM; CHROMAFFIN CELL; EXOCYTOSIS; TETANUS TOXIN;
D O I
10.1111/j.1471-4159.1992.tb09771.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We compared the characteristics of secretion stimulated by EGTA-buffered Ba2+- and Ca2+-containing solutions in digitonin-permeabilized bovine adrenal chromaffin cells. Half-maximal secretion occurred at approximately 100-mu-M Ba2+ or 1-mu-M Ca2+. Ba2+-stimulated release was not due to release of sequestered intracellular Ca2+ because at a constant free Ba2+ concentration, increasing unbound EGTA did not diminish the extent of release due to Ba2+. The maximal extents of Ba2+- and Ca2+-dependent secretion in the absence of MgATP were identical. MgATP enhanced Ba2+-induced secretion to a lesser extent than Ca2+-induced secretion. Half-maximal concentrations of Ba2+ and Ca2+, when added together to cells, yielded approximately additive amounts of secretion. Maximal concentrations of Ba2+ and Ca2+ when added together to cells for 2 or 15 min were not additive. Tetanus toxin inhibited Ba2+- and Ca2+-dependent secretion to a similar extent. Ba2+, unlike Ca2+, did not activate polyphosphoinositide-specific phospholipase C. These data indicate that (1) Ba2+ directly stimulates exocytosis, (2) Ba2+-induced secretion is stimulated to a lesser extent than Ca2+-dependent secretion by MgATP, (3) Ba2+ and Ca2+ use similar pathways to trigger exocytosis, and (4) exocytosis from permeabilized cells does not require activation of polyphosphoinositide-specific phospholipase C.
引用
收藏
页码:680 / 687
页数:8
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