SELECTIVE DETERMINATION, IN PLASMA, OF ARTEMETHER AND ITS MAJOR METABOLITE, DIHYDROARTEMISININ, BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH ULTRAVIOLET DETECTION

被引：55

作者：

THOMAS, CG

WARD, SA

EDWARDS, G

机构：

[1] UNIV LIVERPOOL,DEPT PHARMACOL & THERAPEUT,LIVERPOOL L69 3BX,ENGLAND

[2] UNIV LIVERPOOL,LIVERPOOL SCH TROP MED,LIVERPOOL L3 5QA,ENGLAND

来源：

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1992年 / 583卷 / 01期

基金：

英国医学研究理事会;

关键词：

D O I：

10.1016/0378-4347(92)80355-T

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A sensitive and selective reversed-phase high-performance liquid chromatographic method for the determination of artemether and its major metabolite dihydroartemisinin in plasma has been developed. It involves extraction of plasma with dichloromethane, solid-phase separation of the two analytes and acid decomposition prior to chromatography on a C18 Spherisorb column with a mobile phase of acetonitrile-water (50:50, v/v). Run time is 30 min. The assay satisfies all of the criteria required for use in clinical pharmacokinetic studies.

引用

页码：131 / 136

页数：6

共 3 条

[1] DETERMINATION OF ARTEETHER IN BLOOD-PLASMA BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH ULTRAVIOLET DETECTION AFTER HYDROLYSIS WITH ACID [J].