ANEUPLOIDY AND POLYPLOIDIZATION IN HAPLOID TISSUE-CULTURES OF LARIX-DECIDUA

被引：0
作者：
VONADERKAS, P
ANDERSON, P
机构：
来源：
PHYSIOLOGIA PLANTARUM | 1993年 / 88卷 / 01期
关键词：
ANEUPLOIDY; CONIFERS; GYNOGENESIS; LARCH; LARIX; POLYPLOIDY;
D O I：
10.1034/j.1399-3054.1993.880110.x
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
Haploid embryogenic lines of Larix decidua which had been maintained on medium free of plant growth regulators were screened for chromosomal abnormalities. Chromosome squashes were prepared from fast-growing suspension culture. Alternatively, a method using burst protoplasts was devised for improving chromosome spreads. One line showed polyploidization with chromosome numbers ranging from haploid to tetraploid. This line is presently predominantly diploid. Aneuploidization also occurred. The most effective method for counting chromosomes was from burst protoplasts. It is recommended that long-term cultures of conifer tissues be checked for genetic stability.
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页码：73 / 77
页数：5
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