SEPARATION OF PROTEINS BY SODIUM DODECYL SULFATE-POLYACRYLAMIDE GEL-ELECTROPHORESIS IN THE PRESENCE OF SOLUBLE, AQUEOUS POLYMERS - FICOLL AND POLYVINYLPYRROLIDONE

In previous studies we have demonstrated that water soluble polymers of dextran and methylcellulose,when incorporated into sodium dodecyl sulfate (SDS)-polyacrylamide gels, can be used to sharpen the protein bands. We have extended these studies to enhance the separations in the mid-molecular weight range. Enhanced separation of protein molecular weight markers between 30 000-67 000 was best achieved in SDS-gels containing 2.5% polyvinylpyrrolidone (average molecular weight 10 000).