REGULATION AND EXPRESSION OF A GROWTH ARREST-SPECIFIC GENE (GAS5) DURING GROWTH, DIFFERENTIATION, AND DEVELOPMENT

被引:206
作者
COCCIA, EM
CICALA, C
CHARLESWORTH, A
CICCARELLI, C
ROSSI, GB
PHILIPSON, L
SORRENTINO, V
机构
[1] EUROPEAN MOLEC BIOL LAB,W-6900 HEIDELBERG,GERMANY
[2] IST SUPER SANITA,VIROL LAB,I-00161 ROME,ITALY
关键词
D O I
10.1128/MCB.12.8.3514
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The growth arrest-specific gas5 gene was isolated from mouse genomic DNA and structurally characterized. The transcriptional unit is divided into 12 exons that span around 7 kb. An alternative splicing mechanism gives rise to two mature mRNAs which contain either 11 or 12 exons, and both are found in the cytoplasm of growth-arrested cells. In vivo, the gas5 gene is ubiquitously expressed in mouse tissues during development and adult life. In Friend leukemia and NIH 3T3 cells, the levels of gas5 gene mRNA were high in saturation density-arrested cells and almost undetectable in actively growing cells. Run-on experiments indicated that the gas5 gene is transcribed at the same level in both growing and arrested cells. On the other hand, in dimethyl sulfoxide-induced differentiating cells a sharp decrease in the rate of transcription was observed shortly before the cells reached the postmitotic stage. These results indicate that in density-arrested cells accumulation of gas5 mRNA is controlled at the posttranscriptional level while in differentiating cells expression is regulated transcriptionally.
引用
收藏
页码:3514 / 3521
页数:8
相关论文
共 49 条
[1]   INTERACTION OF RETROVIRAL ONCOGENES WITH THE DIFFERENTIATION PROGRAM OF MYOGENIC CELLS [J].
ALEMA, S ;
TATO, F .
ADVANCES IN CANCER RESEARCH, 1987, 49 :1-28
[2]   COMPLEXITY OF THE EARLY GENETIC RESPONSE TO GROWTH-FACTORS IN MOUSE FIBROBLASTS [J].
ALMENDRAL, JM ;
SOMMER, D ;
MACDONALDBRAVO, H ;
BURCKHARDT, J ;
PERERA, J ;
BRAVO, R .
MOLECULAR AND CELLULAR BIOLOGY, 1988, 8 (05) :2140-2148
[3]   EXPRESSION OF A MOUSE LONG TERMINAL REPEAT IS CELL CYCLE-LINKED [J].
AUGENLICHT, LH ;
HALSEY, H .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1985, 82 (07) :1946-1949
[4]  
BATTISTINI A, 1991, J BIOL CHEM, V266, P528
[5]   RAPID REPRESSION OF QUIESCENCE-SPECIFIC GENE-EXPRESSION BY EPIDERMAL GROWTH-FACTOR, INSULIN, AND PP60V-SRC [J].
BEDARD, PA ;
YANNONI, Y ;
SIMMONS, DL ;
ERIKSON, RL .
MOLECULAR AND CELLULAR BIOLOGY, 1989, 9 (03) :1371-1375
[6]   REPRESSION OF QUIESCENCE-SPECIFIC POLYPEPTIDES IN CHICKEN HEART MESENCHYMAL CELLS TRANSFORMED BY ROUS-SARCOMA VIRUS [J].
BEDARD, PA ;
BALK, SD ;
GUNTHER, HS ;
MORISI, A ;
ERIKSON, RL .
MOLECULAR AND CELLULAR BIOLOGY, 1987, 7 (04) :1450-1458
[7]   HISTONE GENE SWITCHING IN MURINE ERYTHROLEUKEMIA-CELLS IS DIFFERENTIATION SPECIFIC AND OCCURS WITHOUT LOSS OF CELL-CYCLE REGULATION [J].
BROWN, DT ;
YANG, YS ;
SITTMAN, DB .
MOLECULAR AND CELLULAR BIOLOGY, 1988, 8 (10) :4406-4415
[8]   INDUCER-MEDIATED COMMITMENT OF MURINE ERYTHROLEUKEMIA-CELLS TO DIFFERENTIATION - A MULTISTEP PROCESS [J].
CHEN, ZX ;
BANKS, J ;
RIFKIND, RA ;
MARKS, PA .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1982, 79 (02) :471-475
[9]   REGULATION OF EXPRESSION OF GROWTH ARREST-SPECIFIC GENES IN MOUSE FIBROBLASTS [J].
CICCARELLI, C ;
PHILIPSON, L ;
SORRENTINO, V .
MOLECULAR AND CELLULAR BIOLOGY, 1990, 10 (04) :1525-1529
[10]  
CICCARELLI C, UNPUB