METHIONINE-ALPHA-NAPHTHYL ESTER, A USEFUL CHROMOGENIC SUBSTRATE FOR ESTERPROTEASES OF THE MOUSE SUB-MANDIBULAR GLAND

被引：36

作者：

SCHALLER, E

DEIMLING, OV

机构：

[1] Department of Chemical Pathology, Institute of Pathology, University of Freiburg

来源：

ANALYTICAL BIOCHEMISTRY | 1979年 / 93卷 / 02期

关键词：

D O I：

10.1016/S0003-2697(79)80147-5

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The two aminoacid esters, N-acetyl-l-methionine α-naphthyl ester and N-acetyl-l-alanine α-naphthyl ester have been found to be suitable chromogenic substrates for the demonstration of mouse submandibular esterproteases. Using these substrates, a complex banding pattern of esterproteases was demonstrated by disc electrophoresis of mouse submandibular gland. Of these, protease A, epidermal growth factor binding protein, and the γ-subunits of 7 S nerve growth factor could be identified. © 1979 Academic Press, Inc.

引用

页码：251 / 256

页数：6

共 16 条

[1] SUBMAXILLARY GLAND OF MOUSE - PROPERTIES OF A PURIFIED PROTEIN AFFECTING MUSCLE TISSUE IN VITRO [J].

ATTARDI, DG ;

SCHLESINGER, MJ ;

SCHLESINGER, S .

SCIENCE, 1967, 156 (3779) :1253-+

[2]

BOESMAN M, 1976, ARCH BIOCHEM BIOPHYS, V175, P463, DOI 10.1016/0003-9861(76)90534-8

[3] RADIOIMMUNOASSAY OF EPIDERMAL GROWTH-FACTOR [J].

BYYNY, RL ;

COHEN, S ;

ORTH, DN .

ENDOCRINOLOGY, 1972, 90 (05) :1261-&

[4] TESTOSTERONE EFFECT ON SYNTHETIC RATE OF TWO ESTEROPEPTIDASES IN MOUSE SUBMAXILLARY GLAND [J].

CALISSANO, P ;

ANGELETTI, PU .

BIOCHIMICA ET BIOPHYSICA ACTA, 1968, 156 (01) :51-+

[5] EXPERIMENTAL ANALYSIS OF MOUSE SUBMAXILLARY SALIVARY GLAND IN RELATIONSHIP TO ITS NERVE-GROWTH FACTOR CONTENT [J].

CARAMIA, F ;

LEVIMONTALCINI, R ;

ANGELETTI, PU .

ENDOCRINOLOGY, 1962, 70 (06) :915-+

[6]

EKFORS TO, 1972, ENZYMOLOGIA, V43, P177

[7]

GOMORI G, 1972, INT REV CYTOL, V1, P324

[8] SUBUNIT INTERACTION AND ENZYMATIC ACTIVITY OF MOUSE 7S NERVE GROWTH FACTOR [J].

GREENE, LA ;

SHOOTER, EM ;

VARON, S .

BIOCHEMISTRY, 1969, 8 (09) :3735-+

[9]

POPP RA, 1962, J HERED, V53, P111

[10] PROTEOLYTIC-ENZYMES FROM MOUSE SUBMAXILLARY-GLAND - SPECIFICITY RESTRICTED TO ARGININE RESIDUES .2. [J].

SCHENKEIN, I ;

LEVY, M ;

FRANKLIN, EC ;

FRANGIONE, B .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1977, 182 (01) :64-70

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