PITUITARY ADENYLATE-CYCLASE ACTIVATING POLYPEPTIDE (PACAP) AND VASOACTIVE-INTESTINAL-PEPTIDE STIMULATE 2 SIGNALING PATHWAYS IN CHO CELLS STABLY TRANSFECTED WITH THE SELECTIVE TYPE-I PACAP RECEPTOR

被引：35

作者：

DELPORTE, C ^{[1
]}

POLOCZEK, P ^{[1
]}

DENEEF, P ^{[1
]}

VERTONGEN, P ^{[1
]}

CICCARELLI, E ^{[1
]}

SVOBODA, M ^{[1
]}

HERCHUELZ, A ^{[1
]}

WINAND, J ^{[1
]}

ROBBERECHT, P ^{[1
]}

机构：

[1] FREE UNIV BRUSSELS,SCH MED,PHARMACOL LAB,BRUSSELS,BELGIUM

来源：

MOLECULAR AND CELLULAR ENDOCRINOLOGY | 1995年 / 107卷 / 01期

关键词：

PITUITARY ADENYLATE CYCLASE ACTIVATING POLYPEPTIDE (PACAP) RECEPTOR CYTOSOLIC FREE CALCIUM; INOSITOL PHOSPHATE; CYCLIC AMP; CHINESE HAMSTER OVARY CELLS;

D O I：

10.1016/0303-7207(94)03424-R

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The properties of the pituitary adenylate cyclase activating polypeptide (PACAP) type I receptor were studied on a clone of Chinese hamster ovary cells (CHO) stably transfected with the recombinant receptor. PACAP(1-27), PACAP(1-38) and VIP inhibited [I-125-acetyi-His(1)]PACAP(1-27) binding, stimulated cyclic AMP and inositol phosphates production and induced [Ca2+](i) increase with the same order of potency: PACAP(1-27)=PACAP(1-38) > VIP. The concentrations required for half maximal receptor occupancy, IP3- and [Ca2+](i) increase were not different for both PACAPs (1 nM) and 100-fold higher than those required for cyclic AMP increase (0.010 nM). These data suggest that the occupancy of a portion of the total receptors available was sufficient for maximal cyclic AMP production but not for maximal IP3 production. It is concluded that the possibility of the type I PACAP receptor being coupled to a transduction pathway is not located at the level of the ligand but rather at the level of the G-proteins.

引用

页码：71 / 76

页数：6

共 29 条

[1] THE NOVEL VIP-LIKE HYPOTHALAMIC POLYPEPTIDE PACAP INTERACTS WITH HIGH-AFFINITY RECEPTORS IN THE HUMAN NEUROBLASTOMA CELL-LINE NB-OK
CAUVIN, A
BUSCAIL, L
GOURLET, P
DENEEF, P
GOSSEN, D
ARIMURA, A
MIYATA, A
COY, DH
ROBBERECHT, P
CHRISTOPHE, J
[J]. PEPTIDES, 1990, 11 (04) : 773 - 777
[2] CHOI OH, 1992, J PHARMACOL EXP THER, V260, P369
[3] DHFR COAMPLIFICATION OF T-PA IN DHFR+ BOVINE ENDOTHELIAL-CELLS - INVITRO CHARACTERIZATION OF THE PURIFIED SERINE PROTEASE
CONNORS, RW
SWEET, RW
NOVERAL, JP
PFARR, DS
TRILL, JJ
SHEBUSKI, RJ
BERKOWITZ, BA
WILLIAMS, D
FRANKLIN, S
REFF, ME
[J]. DNA-A JOURNAL OF MOLECULAR & CELLULAR BIOLOGY, 1988, 7 (09): : 651 - 661
[4] SELECTIVE EFFECTS OF MASTOPARAN ANALOGS - SEPARATION OF G-PROTEIN-DIRECTED AND MEMBRANE-PERTURBING ACTIVITIES
DANILENKO, M
WORLAND, P
CARLSON, B
SAUSVILLE, EA
SHARONI, Y
[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1993, 196 (03) : 1296 - 1302
[5] CONTRASTING EFFECTS OF PACAP AND CARBACHOL ON [CA-2+]I AND INOSITOL PHOSPHATES IN HUMAN NEUROBLASTOMA NB-OK-1 CELLS
DELPORTE, C
VANPRAET, A
HERCHUELZ, A
WINAND, J
CHRISTOPHE, J
[J]. PEPTIDES, 1993, 14 (06) : 1111 - 1118
[6] ATRIAL-NATRIURETIC-PEPTIDE BINDS TO ANP-R(1) RECEPTORS IN NEUROBLASTOMA-CELLS OR IS DEGRADED EXTRACELLULARLY AT THE SER-PHE BOND
DELPORTE, C
POLOCZEK, P
TASTENOY, M
WINAND, J
CHRISTOPHE, J
[J]. EUROPEAN JOURNAL OF PHARMACOLOGY-MOLECULAR PHARMACOLOGY SECTION, 1992, 227 (03): : 247 - 256
[7] DEUTSCH PJ, 1992, J BIOL CHEM, V267, P5108
[8] FRAKER PJ, 1978, BIOCHEM BIOPH RES CO, V80, P849, DOI 10.1016/0006-291X(78)91322-0
[9] CHARACTERIZATION AND DISTRIBUTION OF BINDING-SITES FOR THE HYPOTHALAMIC PEPTIDE, PITUITARY ADENYLATE CYCLASE-ACTIVATING POLYPEPTIDE
GOTTSCHALL, PE
TATSUNO, I
MIYATA, A
ARIMURA, A
[J]. ENDOCRINOLOGY, 1990, 127 (01) : 272 - 277
[10] MOLECULAR-CLONING AND FUNCTIONAL EXPRESSION OF RAT CDNAS ENCODING THE RECEPTOR FOR PITUITARY ADENYLATE-CYCLASE ACTIVATING POLYPEPTIDE (PACAP)
HOSOYA, M
ONDA, H
OGI, K
MASUDA, Y
MIYAMOTO, Y
OHTAKI, T
OKAZAKI, H
ARIMURA, A
FUJINO, M
[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1993, 194 (01) : 133 - 143

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