CLONING AND SEQUENCE-ANALYSIS OF AN ESTERASE GENE FROM PSEUDOMONAS SP KWI-56

被引：15
作者：
SHIMADA, Y
NAGAO, T
SUGIHARA, A
IIZUMI, T
YUI, T
NAKAMURA, K
FUKASE, T
TOMINAGA, Y
机构：
[1] KURITA WATER IND LTD,KURITA CENT LAB,KANAGAWA,JAPAN
[2] MIYAZAKI UNIV,FAC ENGN,DEPT MAT SCI,MIYAZAKI 880,JAPAN
来源：
BIOCHIMICA ET BIOPHYSICA ACTA | 1993年 / 1174卷 / 01期
关键词：
ESTERASE; NUCLEOTIDE SEQUENCE; CLONING; SEQUENCE ANALYSIS; (PSEUDOMONAS);
D O I：
10.1016/0167-4781(93)90095-U
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The gene encoding an esterase from Pseudomonas sp. KWI-56 was cloned and sequenced. The nucleotide sequence contained an open reading frame encoding a polypeptide comprising 262 amino acids, whose molecular weight agreed well with the value obtained by SDS-PAGE. Comparison of the amino acid sequence with those of the other homologous enzymes suggested that Ser-92 and His-241 might be included in the catalytic triad.
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页码：79 / 82
页数：4
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