LYSIS AND FRACTIONATION OF MYCOBACTERIUM-PARATUBERCULOSIS AND ESCHERICHIA-COLI BY MATRIX SOLID-PHASE DISPERSION

被引：15

作者：

HINES, ME

LONG, AR

SNIDER, TG

BARKER, SA

机构：

[1] LOUISIANA STATE UNIV,SCH VET MED,DEPT VET PHYSIOL PHARMACOL & TOXICOL,BATON ROUGE,LA 70803

[2] LOUISIANA STATE UNIV,SCH VET MED,DEPT VET PATHOL,BATON ROUGE,LA 70803

[3] LOUISIANA STATE UNIV,SCH VET MED,ANALYT SYST LAB,BATON ROUGE,LA 70803

来源：

ANALYTICAL BIOCHEMISTRY | 1991年 / 195卷 / 02期

关键词：

D O I：

10.1016/0003-2697(91)90317-M

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A novel method for the lysis and subsequent fractionation of bacterial constituents from Mycobacterium paratuberculosis strain 19698 (M. paratuberculosis) and Escherichia coli strain DH5α utilizing the technique of matrix solid-phase dispersion (MSPD) is described. Bacteria were blended with octadecylsilyl (C18) derivatized silica to obtain cellular lysis. The blended material was used to prepare a column which was sequentially eluted with solvents of increasing polarity. Fractionation of cellular components was confirmed by analysis of the solvent extracts. The possible applicability of the MSPD technique as a general method for the lysis and fractionation of bacterial components is proposed. © 1991.

引用

页码：197 / 206

页数：10

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