EFFECTS OF ANTICOAGULANTS AND STORAGE OF BLOOD-SAMPLES ON EFFICACY OF THE POLYMERASE CHAIN-REACTION ASSAY FOR HEPATITIS-C VIRUS

被引：115

作者：

WANG, JT

WANG, TH

SHEU, JC

LIN, SM

LIN, JT

CHEN, DS

机构：

[1] NATL TAIWAN UNIV HOSP,DEPT INTERNAL MED,TAIPEI 100,TAIWAN

[2] NATL TAIWAN UNIV HOSP,HEPATITIS RES CTR,TAIPEI 100,TAIWAN

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 1992年 / 30卷 / 03期

关键词：

D O I：

10.1128/JCM.30.3.750-753.1992

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Blood samples from 11 patients with posttransfusion hepatitis C virus infection were collected. Each sample was divided into three fractions to obtain sera, sodium-citrated plasma, and heparinized plasma and then tested for HCV RNA by a nested polymerase chain reaction (PCR). Of them, eight sodium-citrated plasma samples, seven serum samples, and no heparinized plasma samples were PCR positive. Eight PCR-positive sodium-citrated plasma samples were exposed to different physical conditions and semiquantified for HCV RNA after serial dilutions. Samples stored at -70-degrees-C showed the best preservation of HCV RNA, and storage at the other conditions resulted in only minimal loss of the PCR signal. Therefore, serum or sodium-citrated plasma specimens are satisfactory for detecting HCV RNA by PCR, but heparinized blood specimens are not.

引用

页码：750 / 753

页数：4

共 18 条

[1] DETECTION OF ANTIBODY TO HEPATITIS-C VIRUS IN PROSPECTIVELY FOLLOWED TRANSFUSION RECIPIENTS WITH ACUTE AND CHRONIC NON-A-HEPATITIS, NON-B-HEPATITIS [J].

ALTER, HJ ;

PURCELL, RH ;

SHIH, JW ;

MELPOLDER, JC ;

HOUGHTON, M ;

CHOO, QL ;

KUO, G .

NEW ENGLAND JOURNAL OF MEDICINE, 1989, 321 (22) :1494-1500

[2]

BEUTLER E, 1990, BIOTECHNIQUES, V9, P166

[3] HEPATITIS-C VIRUS-INFECTION IN AN AREA HYPERENDEMIC FOR HEPATITIS-B AND CHRONIC LIVER-DISEASE - THE TAIWAN EXPERIENCE [J].

CHEN, DS ;

KUO, GC ;

SUNG, JL ;

LAI, MY ;

SHEU, JC ;

CHEN, PJ ;

YANG, PM ;

HSU, HM ;

CHANG, MH ;

CHEN, CJ ;

HAHN, LC ;

CHOO, QL ;

WANG, TH ;

HOUGHTON, M .

JOURNAL OF INFECTIOUS DISEASES, 1990, 162 (04) :817-822

[4] DETECTION OF HEPATITIS-C VIRAL SEQUENCES IN BLOOD DONATIONS BY NESTED POLYMERASE CHAIN-REACTION AND PREDICTION OF INFECTIVITY [J].

GARSON, JA ;

TEDDER, RS ;

BRIGGS, M ;

TUKE, P ;

GLAZEBROOK, JA ;

TRUTE, A ;

PARKER, D ;

BARBARA, JAJ ;

CONTRERAS, M ;

ALOYSIUS, S .

LANCET, 1990, 335 (8703) :1419-1422

[5] CHARACTERIZATION OF THE TERMINAL REGIONS OF HEPATITIS-C VIRAL-RNA - IDENTIFICATION OF CONSERVED SEQUENCES IN THE 5' UNTRANSLATED REGION AND POLY(A) TAILS AT THE 3' END [J].

HAN, JH ;

SHYAMALA, V ;

RICHMAN, KH ;

BRAUER, MJ ;

IRVINE, B ;

URDEA, MS ;

TEKAMPOLSON, P ;

KUO, G ;

CHOO, QL ;

HOUGHTON, M .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (05) :1711-1715

[6] INHIBITION OF HUMAN-IMMUNODEFICIENCY-VIRUS GENE AMPLIFICATION BY HEPARIN [J].

HOLODNIY, M ;

KIM, S ;

KATZENSTEIN, D ;

KONRAD, M ;

GROVES, E ;

MERIGAN, TC .

JOURNAL OF CLINICAL MICROBIOLOGY, 1991, 29 (04) :676-679

[7] AN ASSAY FOR CIRCULATING ANTIBODIES TO A MAJOR ETIOLOGIC VIRUS OF HUMAN NON-A, NON-B-HEPATITIS [J].

KUO, G ;

CHOO, QL ;

ALTER, HJ ;

GITNICK, GL ;

REDEKER, AG ;

PURCELL, RH ;

MIYAMURA, T ;

DIENSTAG, JL ;

ALTER, MJ ;

STEVENS, CE ;

TEGTMEIER, GE ;

BONINO, F ;

COLOMBO, M ;

LEE, WS ;

KUO, C ;

BERGER, K ;

SHUSTER, JR ;

OVERBY, LR ;

BRADLEY, DW ;

HOUGHTON, M .

SCIENCE, 1989, 244 (4902) :362-364

[8] AVOIDING FALSE POSITIVES WITH PCR [J].

KWOK, S ;

HIGUCHI, R .

NATURE, 1989, 339 (6221) :237-238

[9]

OKAMOTO H, 1990, JPN J EXP MED, V60, P215

[10]

OKAMOTO H, 1990, JPN J EXP MED, V60, P167

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