DETERMINATION OF ZEARALENONE IN CORN BY LASER FLUORIMETRY

被引：27

作者：

DIEBOLD, GJ ^{[1
]}

KARNY, N ^{[1
]}

ZARE, RN ^{[1
]}

机构：

[1] STANFORD UNIV, DEPT CHEM, STANFORD, CA 94305 USA

来源：

ANALYTICAL CHEMISTRY | 1979年 / 51卷 / 01期

关键词：

D O I：

10.1021/ac50037a024

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

By combining laser fluorimetry with high pressure liquid chromatography, one could detect and quantitate the naturally fluorescent mycotoxin, zearalenone, in contaminated corn samples. Experiments with zearalenone standards show a linear fluorimeter response over 4 orders of magnitude with a detection limit of 300 pg zearalenone injected onto a C18 reverse phase column. The corn samples are first purified using a small silica gel column. The recovery from this step is 86% over the range from 5 ppb to 2.5 ppm. Based on the magnitude of the zearalenone signals compared to the flatness of the base line for zearalenone-free corn samples, a limit of 5 ppb is placed on the detection of zearalenone by this procedure.

引用

页码：67 / 69

页数：3

共 18 条

[1] LASER FLUORESCENCE ANALYSIS OF CHROMATOGRAMS - SUB-NANOGRAM DETECTION OF AFLATOXINS [J].