PROPERTIES AND ANTI-HIV ACTIVITY OF CIRCULAR SENSE AND ANTISENSE OLIGONUCLEOTIDES

被引：5
|
作者：
YAMAKAWA, H
ISHIBASHI, T
NAKASHIMA, H
YAMAMOTO, N
TAKAI, K
TAKAKU, H
机构：
[1] CHIBA INST TECHNOL,DEPT IND CHEM,NARASHINO,CHIBA 275,JAPAN
[2] RAT DRUG DESIGN LABS,FUKUSHIMA 96012,JAPAN
[3] YAMANASHI MED UNIV,DEPT MICROBIOL,YAMANASHI 40938,JAPAN
[4] TOKYO MED & DENT UNIV,SCH MED,DEPT MICROBIOL,BUNKYO KU,TOKYO 113,JAPAN
来源：
NUCLEOSIDES & NUCLEOTIDES | 1995年 / 14卷 / 3-5期
关键词：
D O I：
10.1080/15257779508012551
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The circularization of a 48 mer oligonucleotide was investigated by using enzymatic ligation with T4 DNA ligase. This method yielded 30% circular molecules. The circular oligonucleotides are molecules with no free ends and are therefore more resistant to exonuclease attack. The circular oligonucleotide has remarkably increased Tm values as compared to the nicked and double stranded DNAs. However, the RNase H activity is lower than that with a single stranded DNA. We also describe the anti-HIV activity of a circular oligonucleotide.
引用
收藏
页码：1149 / 1152
页数：4
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