PROGENY ANALYSIS OF TOBACCO THAT EXPRESS A MAMMALIAN DELTA-9 DESATURASE

被引：6

作者：

GRAYBURN, WS

HILDEBRAND, DF

机构：

[1] UNIV KENTUCKY,DEPT AGRON,LEXINGTON,KY 40546

[2] USDA ARS,NO CROP SCI LAB,FARGO,ND 58105

来源：

JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY | 1995年 / 72卷 / 03期

关键词：

FATTY ACIDS; LIPIDS; PALMITIC ACID; VEGETABLE OIL QUALITY;

D O I：

10.1007/BF02541089

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

Various tissues of Nicotiana tabacum plants transformed with a rat stearyl-CoA desaturase gene were analyzed for lipid composition. Gas chromatographic analysis showed a decrease in saturated 16 and 18 carbon fatty acids in leaves and roots of several progeny. Polymerase chain reaction analysis of total DNA from leaves indicated the presence of the desaturase gene in kanamycin-resistant progeny with altered fatty acid composition. Western immunoblot analysis confirmed the presence of introduced desaturase protein. Data on the inheritance of kanamycin resistance is presented.

引用

页码：317 / 321

页数：5

共 13 条

[1]

BUDAR F, 1986, GENETICS, V114, P303

[2]

ENOCH HG, 1976, J BIOL CHEM, V251, P5095

[3] FATTY-ACID ALTERATION BY A DELTA-9 DESATURASE IN TRANSGENIC TOBACCO TISSUE [J].