RETINOIC ACID INHIBITS THE MYRISTOYLATION OF A MEMBRANE-PROTEIN IN HL-60 CELLS

被引：3

作者：

ALMAGOR, M

BARTANA, J

机构：

[1] Department of Biochemistry, Hebrew University - Hadassah Medical School, Jerusalem

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1990年 / 172卷 / 02期

关键词：

D O I：

10.1016/0006-291X(90)90757-E

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Incubation of nondifferentiated HL-60 cells with high specific activity myristic acid results in myristoylation of a 25 KD membrane protein. Myristoylation was inhibited by retinoic acid but not by DMSO, thus indicating that the nonmyristoylated state induced by retinoic acid is causal to rather than the result of the overall differentiation sequel induced. Similarly, incubation of HL-60 cells with high specific activity retinoic acid results in specific retinoylation of a membrane protein of similar molecular mass. The two acylated proteins could however be separated by 2-D gel electrophoresis, thus indicating that inhibition of myristoylation by retinoic acid could not be accounted for by competition between retinoic and myristic acids for the same acylated site. © 1990.

引用

页码：877 / 882

页数：6

共 17 条

[1] INTERFERON-GAMMA INDUCES THE MYRISTOYLATION OF A 48-KDA PROTEIN IN MACROPHAGES [J].