INVITRO TRANSCRIPTION FROM CAULIFLOWER MOSAIC-VIRUS PROMOTERS BY A CELL-FREE-EXTRACT FROM TOBACCO CELLS

被引：24

作者：

COOKE, R

PENON, P

机构：

[1] Laboratoire de Physiologie Végétale (UA565 du CNRS), Université de Perpignan, Perpignan-Cedex, 66025, Avenue de Villeneuve

来源：

PLANT MOLECULAR BIOLOGY | 1990年 / 14卷 / 03期

关键词：

Nicotiana tabacum; promoter; RNA polymerase II; transcription initiation;

D O I：

10.1007/BF00028775

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have studied transcription from the cauliflower mosaic virus 19S and 35S promoters in a cell-free system derived from tobacco cells in suspension culture. While a whole-cell extract is incapable of detectable transcription from these promoters, successive purification by column chromatography allows the preparation of two fractions which contain all factors necessary for transcription from the 19S promoter. In contrast, transcription from the 35S promoter leads to the accumulation of short RNAs. This accumulation can only be partially alleviated by modifying the conditions of transcription. © 1990 Kluwer Academic Publishers.

引用

页码：391 / 405

页数：15

共 27 条

[1] PARTIAL-PURIFICATION OF PLANT TRANSCRIPTION FACTORS .1. INITIATION [J].

ACKERMAN, S ;

FLYNN, PA ;

DAVIS, EA .

PLANT MOLECULAR BIOLOGY, 1987, 9 (02) :147-158

[2]

BIRNBOIM HC, 1979, NUCLEIC ACIDS RES, V7, P1513

[3]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[4] A YEAST ACTIVITY CAN SUBSTITUTE FOR THE HELA-CELL TATA BOX FACTOR [J].

CAVALLINI, B ;

HUET, J ;

PLASSAT, JL ;

SENTENAC, A ;

EGLY, JM ;

CHAMBON, P .

NATURE, 1988, 334 (6177) :77-80

[5] SELECTIVE DINUCLEOTIDE-PRIMED INVITRO TRANSCRIPTION OF A CLONED FRAGMENT OF CAULIFLOWER MOSAIC-VIRUS DNA IS DEPENDENT ON A LIMITED REGION OF THE VIRAL GENOME [J].

COOKE, R ;

PENON, P .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1986, 157 (01) :83-89

[6] DETECTION OF A POTENTIAL TRANSCRIPTION CONTROL SEQUENCE ON THE CAULIFLOWER MOSAIC-VIRUS GENOME BY DINUCLEOTIDE PRIMED INVITRO TRANSCRIPTION [J].

COOKE, R ;

PENON, P .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1986, 138 (01) :17-23

[7] SELECTIVE TRANSCRIPTION OF A CLONED CAULIFLOWER MOSAIC-VIRUS DNA FRAGMENT INVITRO BY SOYBEAN RNA POLYMERASE-II IN THE PRESENCE OF DINUCLEOTIDE PRIMERS [J].

COOKE, RM ;

PENON, P ;

GOT, C ;

MIASSOD, R .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1983, 137 (1-2) :365-371

[8] CHARACTERIZATION OF CAULIFLOWER MOSAIC-VIRUS DNA-SEQUENCES WHICH ENCODE MAJOR POLYADENYLATED TRANSCRIPTS [J].

COVEY, SN ;

LOMONOSSOFF, GP ;

HULL, R .

NUCLEIC ACIDS RESEARCH, 1981, 9 (24) :6735-6747

[9]

COVEY SN, 1985, MOL PLANT VIROLOGY, V2, P121

[10] FORMATION OF STABLE PRE-INITIATION COMPLEXES BETWEEN EUKARYOTIC CLASS-B TRANSCRIPTION FACTORS AND PROMOTER SEQUENCES [J].

DAVISON, BL ;

EGLY, JM ;

MULVIHILL, ER ;

CHAMBON, P .

NATURE, 1983, 301 (5902) :680-686

← 1 2 3 →