SYNTHESIS AND CHARACTERIZATION OF CATIONIC LATEX-PARTICLES BEARING SULFHYDRYL-GROUPS AND THEIR USE IN THE IMMOBILIZATION OF FAB ANTIBODY FRAGMENTS .1.

被引：60

作者：

DELAIR, T ^{[1
]}

PICHOT, C ^{[1
]}

MANDRAND, B ^{[1
]}

机构：

[1] CNRS,UNITE MIXTE BIOMERIEUX,LYON,FRANCE

来源：

COLLOID AND POLYMER SCIENCE | 1994年 / 272卷 / 01期

关键词：

EMULSION POLYMERIZATION; FUNCTIONALIZED PARTICLES; SULFHYDRYL GROUPS; ANTIBODY FRAGMENTS; IMMOBILIZATION;

D O I：

10.1007/BF00653312

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

Emulsion copolymerization of styrene and a cationic monomer vinylbenzyl-isothiouronium chloride (VBIC) with initiation by 2,2' azobis(2-amidinopropane)-dihydrochloride (V-50) gave monodisperse latex particles. After post-stabilization with cationic or non-ionic surfactants, the colloids were diluted in basic buffers with concomitant deprotection of the sulfhydryl groups. Therefore, coupling reactions on the latex beads were possible. Coupling conditions were determined with Ellman's reagent. C-14 iodoacetamide and anti C-reactive protein antibody Fab fragments were immobilized and finally, the immunological activity of the sensitized latex was assessed.

引用

页码：72 / 81

页数：10

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