Relaxation-Based Magic-Angle Spinning NMR Approaches for Studying Protein Dynamics

被引：7
作者：
Lamley, Jonathan M. [1 ]
Lewandowski, Jozef R. [2 ]
机构：
[1] Univ Warwick, Coventry, W Midlands, England
[2] Univ Warwick, Phys Chem, Coventry, W Midlands, England
来源：
EMAGRES | 2016年 / 5卷 / 03期
关键词：
solid-state NMR; relaxation; protein dynamics; magic angle spinning; spin diffusion; molecular motions;
D O I：
10.1002/9780470034590.emrstm1417
中图分类号：
O433 [光谱学];
学科分类号：
0703 ; 070302 ;
摘要：
Solid-state magic angle spinning (MAS) NMR relaxation measurements provide a powerful tool for characterizing protein dynamics. We provide a general introduction to contemporary relaxation-based MAS NMR approaches by reviewing the basic theoretical concepts, experimental considerations, and example procedures for quantitative analysis. We discuss methods suitable for characterization of motions occurring on time scales in the range from picoseconds to milliseconds. We also sketch out strategies to obtain time scales, amplitudes, and directions of molecular motions.
引用
收藏
页码：1423 / 1433
页数：11
相关论文
共 91 条
[1]   De Novo 3D Structure Determination from Sub-milligram Protein Samples by Solid-State 100 kHz MAS NMR Spectroscopy [J].
Agarwal, Vipin ;
Penzel, Susanne ;
Szekely, Kathrin ;
Cadalbert, Riccardo ;
Testori, Emilie ;
Oss, Andres ;
Past, Jaan ;
Samoson, Ago ;
Ernst, Matthias ;
Boeckmann, Anja ;
Meier, Beat H. .
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2014, 53 (45) :12253-12256
[2]   SPIN DIFFUSION AND SPIN-LATTICE RELAXATION OF DEUTERIUM IN ROTATING SOLIDS [J].
ALLA, M ;
ECKMAN, R ;
PINES, A .
CHEMICAL PHYSICS LETTERS, 1980, 71 (01) :148-151
[3]   NUCLEAR MAGNETIC RESONANCE SPECTRA FROM A CRYSTAL ROTATED AT HIGH SPEED [J].
ANDREW, ER ;
BRADBURY, A ;
EADES, RG .
NATURE, 1958, 182 (4650) :1659-1659
[4]   Access to Cα Backbone Dynamics of Biological Solids by 13C T1 Relaxation and Molecular Dynamics Simulation [J].
Asami, Sam ;
Porter, Justin R. ;
Lange, Oliver F. ;
Reif, Bernd .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2015, 137 (03) :1094-1100
[5]   Site-Specific Solid-State NMR Studies of "Trigger Factor" in Complex with the Large Ribosomal Subunit 50S [J].
Barbet-Massin, Emeline ;
Huang, Chih-Ting ;
Daebel, Venita ;
Hsu, Shang-Te Danny ;
Reif, Bernd .
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2015, 54 (14) :4367-4369
[6]   Solid-state NMR of proteins sedimented by ultracentrifugation [J].
Bertini, Ivano ;
Luchinat, Claudio ;
Parigi, Giacomo ;
Ravera, Enrico ;
Reif, Bernd ;
Turano, Paola .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2011, 108 (26) :10396-10399
[7]   ON THE INTERACTION OF NUCLEAR SPINS IN A CRYSTALLINE LATTICE [J].
BLOEMBERGEN, N .
PHYSICA, 1949, 15 (3-4) :386-426
[8]  
BRUSCHWEILER R, 1994, J AM CHEM SOC, V116, P8426
[9]  
Burnham KP, 2002, MODEL SELECTION MULT
[10]   Effects of amantadine on the dynamics of membrane-bound influenza A M2 transmembrane peptide studied by NMR relaxation [J].
Cady, Sarah D. ;
Hong, Mei .
JOURNAL OF BIOMOLECULAR NMR, 2009, 45 (1-2) :185-196
12345678910