CRYOENZYMOLOGY IN AQUEOUS-MEDIA - MICELLAR SOLUBILIZED WATER CLUSTERS

被引：101
作者：
DOUZOU, P [1 ]
KEH, E [1 ]
BALNY, C [1 ]
机构：
[1] INSERM, U128, F-34033 MONTPELLIER, FRANCE
来源：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1979年 / 76卷 / 02期
关键词：
D O I：
10.1073/pnas.76.2.681
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Amphiphilic compounds dissolved in nonpolar organic solvents formed inverted micelles whose aqueous centers can solubilize relatively large amounts of enzymes. The solutions were homogeneous and optically transparent and had low viscosity and freezing points. They provided unique and favorable systems to perform the main cryoenzymologic studies previously carried out in mixed solvents. The preparation and properties of such micelles were described. The absorbance spectra of cytochrome c at various temperatures from -38.degree. C to 20.degree. C were given. The pH dependence of trypsin-catalyzed hydrolysis of benzoylarginine ethyl ester in inverted micelles, as well as kinetic properties and activation energy, was described. Some problems were analyzed.
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页码：681 / 684
页数：4
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