RANDOM AMPLIFIED POLYMORPHIC DNA OF A GROUP OF HALOPHILIC ARCHAEAL ISOLATES

被引：5
作者：
MARTINEZMURCIA, AJ
RODRIGUEZVALERA, F
机构：
[1] Departamento de Genética y Microbiología, Universidad de Alicante, Alicante, 03080, Campus de San Juan
来源：
SYSTEMATIC AND APPLIED MICROBIOLOGY | 1994年 / 17卷 / 03期
关键词：
HALOFERAX MEDITERRANEI; RAPD; TAXONOMY;
D O I：
10.1016/S0723-2020(11)80056-1
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Random amplified polymorphic DNA (RAPD) can be used to characterize closely related bacterial strains. In the present study, we have applied RAPD to some strains of the halophilic archaeon Haloferax mediterranei in an attempt to evaluate the newly developed method and to compare the results with those obtained from pulsed-field gel electrophoresis (PFGE) studies. The RAPD analysis correlated very well with analyses obtained from PFGE. Our results indicate that RAPD is a useful technique to type the total genome with a resolution similar to that of PFGE, but with the advantage of rapidity and simplicity.
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页码：395 / 401
页数：7
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