GENES FOR DIRECTING VACUOLAR MORPHOGENESIS IN SACCHAROMYCES-CEREVISIAE .1. ISOLATION AND CHARACTERIZATION OF 2 CLASSES OF VAM MUTANTS

被引:0
作者
WADA, Y [1 ]
OHSUMI, Y [1 ]
ANRAKU, Y [1 ]
机构
[1] UNIV TOKYO,FAC SCI,DEPT BIOL,BUNKYO KU,TOKYO 113,JAPAN
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中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We identified nine VAM genes (for vacuolar morphology) by genetic analyses on mutants with defective vacuolar morphologies and assembly in the yeast Saccharomyces cerevisiae. The nine VAM genes were classified into two classes according to the mutant phenotypes. The class I vam mutants (vam1, vam5, vam8, and vam9) show a few small vesicles that are stained with histochemical markers for the vacuolar compartment. They also have defects in the maturation of vacuolar marker proteins, and their growth is hypersensitive to high concentrations of CaCl2 or a temperature of 37-degrees-C. There are apparent genetic overlaps among the class I vam mutations and other mutations including cls, end, pep, and vps, which have been shown to be involved in the expression of the vacuolar functions. The class II vam mutants (vam2, vam3, vam4, vam6, and vam7) contain numerous small vesicles stained with the vacuolar histochemical markers and mature forms of the vacuolar proteins and do not show any apparent growth defects in the presence of CaCl2 or at 37-degrees-C.
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页码:18665 / 18670
页数:6
相关论文
共 41 条
[1]   PEP4 GENE OF SACCHAROMYCES-CEREVISIAE ENCODES PROTEINASE-A, A VACUOLAR ENZYME REQUIRED FOR PROCESSING OF VACUOLAR PRECURSORS [J].
AMMERER, G ;
HUNTER, CP ;
ROTHMAN, JH ;
SAARI, GC ;
VALLS, LA ;
STEVENS, TH .
MOLECULAR AND CELLULAR BIOLOGY, 1986, 6 (07) :2490-2499
[2]   STRUCTURE AND FUNCTION OF THE YEAST VACUOLAR MEMBRANE PROTON ATPASE [J].
ANRAKU, Y ;
UMEMOTO, N ;
HIRATA, R ;
WADA, Y .
JOURNAL OF BIOENERGETICS AND BIOMEMBRANES, 1989, 21 (05) :589-603
[3]  
ANRAKU Y, 1987, BIOENERGETICS STRUCT, P249
[4]  
ANRAKU Y, 1991, NEW ERA BIOENERGETIC, P133
[5]   ISOLATION OF YEAST MUTANTS DEFECTIVE IN PROTEIN TARGETING TO THE VACUOLE [J].
BANKAITIS, VA ;
JOHNSON, LM ;
EMR, SD .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1986, 83 (23) :9075-9079
[6]   ORGANELLE ASSEMBLY IN YEAST - CHARACTERIZATION OF YEAST MUTANTS DEFECTIVE IN VACUOLAR BIOGENESIS AND PROTEIN SORTING [J].
BANTA, LM ;
ROBINSON, JS ;
KLIONSKY, DJ ;
EMR, SD .
JOURNAL OF CELL BIOLOGY, 1988, 107 (04) :1369-1383
[7]   ANILINE BLUE AND FLUORESCENCE MICROSCOPY OF CALLOSE IN BULB SCALES OF ALLIUM-CEPA L [J].
CURRIER, HB ;
STRUGGER, S .
PROTOPLASMA, 1956, 45 (04) :552-559
[8]  
KAKINUMA Y, 1981, J BIOL CHEM, V256, P859
[9]   DYNAMIC ASPECTS OF VACUOLAR AND CYTOSOLIC AMINO-ACID POOLS OF SACCHAROMYCES-CEREVISIAE [J].
KITAMOTO, K ;
YOSHIZAWA, K ;
OHSUMI, Y ;
ANRAKU, Y .
JOURNAL OF BACTERIOLOGY, 1988, 170 (06) :2683-2686
[10]   MUTANTS OF SACCHAROMYCES-CEREVISIAE WITH DEFECTIVE VACUOLAR FUNCTION [J].
KITAMOTO, K ;
YOSHIZAWA, K ;
OHSUMI, Y ;
ANRAKU, Y .
JOURNAL OF BACTERIOLOGY, 1988, 170 (06) :2687-2691