A NEW ACRYLIC RESIN FORMULATION - A USEFUL TOOL FOR HISTOLOGICAL, ULTRASTRUCTURAL, AND IMMUNOCYTOCHEMICAL INVESTIGATIONS

被引:205
作者
SCALA, C
CENACCHI, G
FERRARI, C
PASQUINELLI, G
PREDA, P
MANARA, GC
机构
[1] UNIV PARMA,CTR MICROSCOPIE ELECTR,I-43100 PARMA,ITALY
[2] UNIV PARMA,IST CLIN DERMATOL,I-43100 PARMA,ITALY
关键词
ACRYLIC HYDROPHILIC RESIN; AROMATIC CROSS-LINKER; HISTOLOGICAL STAININGS; IMMUNOHISTOCHEMISTRY; ULTRASTRUCTURE; IMMUNOELECTRON MICROSCOPY;
D O I
10.1177/40.11.1431065
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
We describe a new formulation for a hydrophilic resin, mostly composed of glycol methacrylate and hydroxypropyl methacrylate and here referred to as bioacryl, that allows the performance of morphological and immunohistochemical investigations at both light and electron microscopic levels. Immunolocalizations performed on bioacryl-embedded tissues are characterized by high specificity with virtually absent background staining. Finally, the new resin yields satisfactory fine-structural preservation, resulting in ultrastructural images of better quality than those obtained with Lowicryl K4M.
引用
收藏
页码:1799 / 1804
页数:6
相关论文
共 22 条
[1]   LOW-TEMPERATURE EMBEDDING IN GLYCOL METHACRYLATE FOR ENZYME-HISTOCHEMISTRY IN PLANT AND ANIMAL TISSUES [J].
ASHFORD, AE ;
ALLAWAY, WG ;
MCCULLY, ME .
JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, 1972, 20 (12) :986-990
[2]   TEMPERATURE CONTROL IN LOWICRYL K4M AND GLYCOL METHACRYLATE DURING POLYMERIZATION - IS THERE A LOW-TEMPERATURE EMBEDDING METHOD [J].
ASHFORD, AE ;
ALLAWAY, WG ;
GUBLER, F ;
LENNON, A ;
SLEEGERS, J .
JOURNAL OF MICROSCOPY, 1986, 144 :107-126
[3]   EFFECT OF TISSUE PROCESSING ON COLLOIDAL GOLD CYTOCHEMISTRY [J].
BENDAYAN, M ;
NANCI, A ;
KAN, FWK .
JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, 1987, 35 (09) :983-996
[4]   RESIN DEVELOPMENT FOR ELECTRON-MICROSCOPY AND AN ANALYSIS OF EMBEDDING AT LOW-TEMPERATURE [J].
CARLEMALM, E ;
GARAVITO, RM ;
VILLIGER, W .
JOURNAL OF MICROSCOPY-OXFORD, 1982, 126 (MAY) :123-143
[5]  
CARLEMALM E, 1988, BIOTECHNOLOGY BIOAPP, P233
[6]   LR WHITE RESIN AND IMPROVED ON-GRID IMMUNOGOLD DETECTION OF VICILIN, A PEA SEED STORAGE PROTEIN [J].
CRAIG, S ;
MILLER, C .
CELL BIOLOGY INTERNATIONAL REPORTS, 1984, 8 (10) :879-886
[7]   POTENTIAL OF IMMUNOGOLD SILVER STAINING FOR THE STUDY OF LEUKOCYTE SUBPOPULATIONS AS DEFINED BY MONOCLONAL-ANTIBODIES [J].
DEWAELE, M ;
DEMEY, J ;
RENMANS, W ;
LABEUR, C ;
JOCHMANS, K ;
VANCAMP, B .
JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, 1986, 34 (10) :1257-1263
[8]   CONTROLLED NUCLEATION FOR REGULATION OF PARTICLE-SIZE IN MONODISPERSE GOLD SUSPENSIONS [J].
FRENS, G .
NATURE-PHYSICAL SCIENCE, 1973, 241 (105) :20-22
[9]   STAINING SECTIONS OF WATER-MISCIBLE RESINS .1. EFFECTS OF THE MOLECULAR-SIZE OF THE STAIN, AND OF RESIN CROSS-LINKING, ON THE STAINING OF GLYCOL METHACRYLATE EMBEDDED TISSUES [J].
GERRITS, PO ;
HOROBIN, RW ;
WRIGHT, DJ .
JOURNAL OF MICROSCOPY-OXFORD, 1990, 160 :279-290
[10]   NEW EMBEDDING MEDIUM FOR ELECTRON MICROSCOPY [J].
GLAUERT, AM ;
ROGERS, GE ;
GLAUERT, RH .
NATURE, 1956, 178 (4537) :803-803