Changes in the Dermal Structure during Cultured Epidermal Autograft Engraftment Process

被引：5

作者：

Hayashi, Minoru ^{[1
]}

Muramatsu, Hideyuki ^{[3
]}

Nakano, Minoru ^{[2
]}

Yamamoto, Naoto ^{[4
]}

Tokunaka, Ryohei ^{[1
]}

Umezawa, Kazuya ^{[1
]}

Hamajima, Akito ^{[5
]}

Araki, Natsue ^{[5
]}

Yoshimoto, Shinya ^{[3
]}

机构：

[1] Japanese Maebashi Red Cross Hosp, Dept Plast & Reconstruct Surg, Gunma, Japan

[2] Japanese Maebashi Red Cross Hosp, Adv Med Emergency & Crit Care Ctr, Gunma, Japan

[3] Showa Univ, Sch Med, Dept Plast & Reconstruct Surg, Tokyo, Japan

[4] New Tokyo Hosp, Dept Plast & Reconstruct Surg, Chiba, Japan

[5] Gunma Childrens Med Ctr, Dept Plast & Reconstruct Surg, Gunma, Japan

来源：

PLASTIC AND RECONSTRUCTIVE SURGERY-GLOBAL OPEN | 2016年 / 4卷 / 09期

关键词：

D O I：

10.1097/GOX.0000000000000838

中图分类号：

R61 [外科手术学];

学科分类号：

摘要：

Background: The use of cultured epithelial autografts for the treatment of extensive burn wounds has become popular in recent years. We examined extensive burn wounds in 14 patients by using a combination of autograft and cultured epithelial autografts developed in Japan (JACE). Methods: We undertook a skin biopsy at 2, 4, and 6 weeks after transplantation with JACE. By using electron microscopy we observed the engraftment process. Results: In transmission electron microscope findings, we recognized the engraftment process of JACE. Keratinocytes matured gradually. Collagen fibers formed thick bundles in the dermis layer. In scanning electron microscope findings, we observed papillary dermis development on the artificial dermis. Conclusions: After managing wound bed preparation by using artificial dermis, we were able to recognize the good result of grafting JACE on meshed 6:1 split thickness autografts. This is because the auto dermis from autograft extended under the JACE, binding between JACE, and the dermis became strong.

引用

页数：7

共 30 条

[11]

HANAICHI T, 1986, J ELECTRON MICROSC, V35, P304

[12] Experience of Using Cultured Epithelial Autografts for the Extensive Burn Wounds in Eight Patients [J].

Hayashi, Minoru ;

Muramatsu, Hideyuki ;

Nakano, Minoru ;

Ito, Hideaki ;

Inoie, Masukazu ;

Tomizuka, Yosuke ;

Inoue, Mayuko ;

Yoshimoto, Shinya .

ANNALS OF PLASTIC SURGERY, 2014, 73 (01) :25-29

[13] A COMPARISON OF CONSERVATIVE VERSUS EARLY EXCISION - THERAPIES IN SEVERELY BURNED PATIENTS [J].

HERNDON, DN ;

BARROW, RE ;

RUTAN, RL ;

RUTAN, TC ;

DESAI, MH ;

ABSTON, S .

ANNALS OF SURGERY, 1989, 209 (05) :547-553

[14] Accelerated tissue regeneration through incorporation of basic fibroblast growth factor-impregnated gelatin microspheres into artificial dermis [J].

Kawai, K ;

Suzuki, S ;

Tabata, Y ;

Ikada, Y ;

Nishimura, Y .

BIOMATERIALS, 2000, 21 (05) :489-499

[15] Outcomes of sprayed cultured epithelial autografts for full-thickness wounds: A single-centre experience [J].

Lee, Haguen .

BURNS, 2012, 38 (06) :931-936

[16] RE-FREEZE DRIED BILAYER ARTIFICIAL SKIN [J].

MATSUDA, K ;

SUZUKI, S ;

ISSHIKI, N ;

IKADA, Y .

BIOMATERIALS, 1993, 14 (13) :1030-1035

[17] ONTOGENY OF THE BASEMENT-MEMBRANE ZONE AFTER GRAFTING CULTURED HUMAN EPITHELIUM - A MORPHOLOGICAL AND IMMUNOELECTRON MICROSCOPIC STUDY [J].

MOMMAAS, AM ;

TEEPE, RGC ;

LEIGH, IM ;

MULDER, AA ;

KOEBRUGGE, EJ ;

VERMEER, BJ .

JOURNAL OF INVESTIGATIVE DERMATOLOGY, 1992, 99 (01) :71-77

[18] CULTURED EPIDERMIS FOR THE COVERAGE OF MASSIVE BURN WOUNDS - A SINGLE CENTER EXPERIENCE [J].

MUNSTER, AM ;

WEINER, SH ;

SPENCE, RJ .

ANNALS OF SURGERY, 1990, 211 (06) :676-680

[19]

OCONNOR NE, 1981, LANCET, V1, P75

[20] Outcome measures in burn care - Is mortality dead? [J].

Pereira, C ;

Murphy, K ;

Herndon, D .

BURNS, 2004, 30 (08) :761-771

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