ISOLATION AND CHARACTERIZATION OF A GLYCOSPHINGOLIPID HAVING A NEW SIALIC ACID

被引：84

作者：

HAKOMORI, SI

SAITO, T

机构：

[1] Department of Preventive Medicine, University of Washington, School of Medicine, Seattle

来源：

BIOCHEMISTRY | 1969年 / 8卷 / 12期

关键词：

D O I：

10.1021/bi00840a061

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A glycosphingolipid, having a carbohydrate structure similar to that of hematoside but with a higher migration rate on thin-layer chromatography, was isolated from equine erythrocyte membranes. The sialosyl residue of this glycolipid was not hydrolyzed by neuraminidase. The sialic acid, isolated from the acid hydrolysate, was identified as a new sialic acid, O-acetyl((N-glycolyl)neuraminic acid. After removal of O-acetyl group, the sialic acid of this glycolipid was hydrolyzable by neuraminidase and its migration rate on thinlayer chromatography was similar to that of known hematoside. The neuraminidase resistant property of the O-acetyl-(N-glycolyl)neuraminosyl was discussed. © 1969, American Chemical Society. All rights reserved.

引用

页码：5082 / &

共 41 条

[1] THE DETERMINATION OF FREE SIALIC ACID IN THE PRESENCE OF THE BOUND COMPOUND [J].