STUDIES ON STRUCTURE OF HUMAN ALPHA2-MACROGLOBULIN .I. PREPARATION OF HUMAN ALPHA2-MACROGLOBULIN

被引：17

作者：

RAZAFIMA.E

BOURRILL.R

机构：

[1] Laboratoire de Biochimie, Faculté de Médecine, Paris

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1968年 / 154卷 / 02期

关键词：

D O I：

10.1016/0005-2795(68)90054-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A method for the large-scale preparation of homogeneous human α2-macroglobulin (α2-M) is described. This technic involves three steps: fractionated precipitation by (NH4)2SO4 (between 1.6 and 1.92 M at pH 6), preparative zone electrophoresis with a Porath Electrophoresis Column, and gel filtration on a column of Sephadex G-200. The homogeneity of this preparation has been checked by ultracentrifugation, electrophoresis on starch gel and immunoelectrophoresis. The trypsin-inhibiting capacity is preserved through the step of purification of human α2-M, the total yield of which is about 75%. The reversible formation of dissociation product 11-12 S in some preparations is discussed. © 1968.

引用

页码：397 / &

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