DICHLOROMETHANE AS AN ECONOMIC-ALTERNATIVE TO CHLOROFORM IN THE EXTRACTION OF DNA FROM PLANT-TISSUES

被引：13

作者：

CHAVES, AL ^{[1
]}

VERGARA, CE ^{[1
]}

MAYER, JE ^{[1
]}

机构：

[1] CTR INT AGR TROP,BIOTECHNOL RES UNIT,CALI,COLOMBIA

来源：

PLANT MOLECULAR BIOLOGY REPORTER | 1995年 / 13卷 / 01期

关键词：

DNA EXTRACTION; DICHLOROMETHANE; PLANT TISSUES;

D O I：

10.1007/BF02668389

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Processing of large numbers samples of plant tissue samples for molecular mapping and gene tagging requires methods that are quick, simple, and cheap, and that eventually carl be automated. Organic solvents used for DNA extraction can represent a significant proportion of the overall cost. In this study we examined dichloromethane as a replacement for chloroform to be used in combination with phenol.

引用

页码：18 / 25

页数：8

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