CLONING AND CHARACTERIZATION OF THE SCHIZOSACCHAROMYCES-POMBE RAD8 GENE, A MEMBER OF THE SNF2 HELICASE FAMILY

被引：27
作者：
DOE, CL
MURRAY, JM
SHAYEGHI, M
HOSKINS, M
LEHMANN, AR
CARR, AM
WATTS, FZ
机构：
[1] UNIV SUSSEX,SCH BIOL SCI,BRIGHTON BN1 9QG,E SUSSEX,ENGLAND
[2] UNIV SUSSEX,MRC,CELL MUTAT UNIT,BRIGHTON BN1 9RR,E SUSSEX,ENGLAND
来源：
NUCLEIC ACIDS RESEARCH | 1993年 / 21卷 / 25期
基金：
英国惠康基金;
关键词：
D O I：
10.1093/nar/21.25.5964
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The Schizosaccharomyces pombe rad8 mutant is sensitive to both UV and gamma irradiation. We have cloned the rad8 gene by complementation of the UV sensitivity of a rad8.190 mutant strain. The gene comprises an open reading frame of 3.4 kb which does not contain any introns and is capable of encoding a 1133 amino acid protein of 129 kDa. Deletion of the gene indicates that it is not essential for cell viability. Recognisable motifs are present for a nuclear localisation signal, a RING finger and helicase domains. The predicted protein is a member of the SNF2 subfamily of proteins and shows particular homology to the Saccharomyces cerevisiae RADS protein. Double mutant analysis demonstrated that the rad8 mutant is not epistatic to mutants in the excision repair pathway (rad13) or checkpoint pathway (rad9). Analysis of radiation sensitivity though the cell cycle indicates that, unlike most other rad mutants, rad8 is most sensitive to irradiation during the G1/S period.
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页码：5964 / 5971
页数：8
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