CLONING OF LYMPHOCYTES-T FROM BRONCHOALVEOLAR LAVAGE FLUID

被引:20
作者
HOL, BEA
KROUWELS, FH
BRUINIER, B
REIJNEKE, RMR
MENGELERS, HJJ
KOENDERMAN, L
JANSEN, HM
OUT, TA
机构
[1] UNIV AMSTERDAM, ACAD MED CTR, DEPT PULM, F4-206, MEIBERGDREEF 9, 1105 AZ AMSTERDAM, NETHERLANDS
[2] UNIV AMSTERDAM, ACAD MED CTR, CLIN IMMUNOL LAB, 1105 AZ AMSTERDAM, NETHERLANDS
[3] CLB, EXPTL & CLIN IMMUNOL LAB, AMSTERDAM, NETHERLANDS
[4] UNIV UTRECHT HOSP, DEPT PULM, 3511 GV UTRECHT, NETHERLANDS
关键词
D O I
10.1165/ajrcmb/7.5.523
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We have prepared T-cell clones from bronchoalveolar lavage fluid (BALF) from four healthy, nonsmoking persons and from four patients with allergic asthma. T cells were cloned by direct limiting dilution and with the use of a fluorescent activated cell sorter with an automated cell deposition unit. T-cell clones from the blood (PB) were prepared as well. The cloning efficiencies of T cells from BALF ranged from 3 to 40% and were lower than those obtained from PB T cells (18 to 72 %). The cloning conditions generated CD4+ as well as CD8+ clones. The very late antigen-4, VLA-4, was more frequently expressed on CD4+ T-cell clones from BALF than from the blood (P < 0.05). CD8+ clones from BALF were more frequently VLA-1+ than those from blood (P < < 0.01). Mitogen- and monoclonal antibody-driven proliferation of CD4+ clones showed that BALF clones were well responsive to proliferation stimuli similar to those from the blood. Analysis of interleukin-4 production by 10 BALF and 10 PB clones showed large variations between individual CD4+ clones (BALF: range, < 100 to 700 pg/ml; PB: range, < 100 to 1,100 pg/ml), indicating the generation of different types of clones, which was also clear from analysis of interferon-gamma production. The analysis of properties of BALF T-cell clones and their regulation will improve insight into immunologic reactions in the lungs.
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页码:523 / 530
页数:8
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