MOLECULAR-CLONING AND CHARACTERIZATION OF A GERMAN HIV-1 ISOLATE

被引：34
作者：
SAUERMANN, U
SCHNEIDER, J
MOUS, J
BRUNCKHORST, U
SCHEDEL, I
JENTSCH, KD
HUNSMANN, G
机构：
[1] DEUTSCH PRIMATENZENTRUM GMBH,VIROL & IMMUNOL ABT,KELLNERWEG 4,W-3400 GOTTINGEN,GERMANY
[2] F HOFFMANN LA ROCHE & CO LTD,CH-4002 BASEL,SWITZERLAND
[3] HANOVER MED SCH,W-3000 HANOVER 61,GERMANY
来源：
AIDS RESEARCH AND HUMAN RETROVIRUSES | 1990年 / 6卷 / 06期
关键词：
D O I：
10.1089/aid.1990.6.813
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
The genetic diversity of HIV-1 is well documented. Except for the HIV-1 isolate LAV-1BRU, no nucleic acid sequence of a European isolate of HIV-1 has been published to date. To further investigate the extent of the genetic variability and the evolution of HIV-1, we have isolated, cloned, and subsequently sequenced HIV-1 from a German patient with AIDS-related complex. Comparative studies of the nucleic acid sequence revealed that this isolate, designated HAN2, is highly divergent from the North American and African subtypes of HIV-1 and may represent a European subtype of HIV-1. Furthermore, a full-length molecular clone was derived from this isolate which was infectious in human T-cell lines. Therefore this new isolate will be particularly useful for studies on the genetic evolution and biology of HIV-1 as well as for testing antiviral substances and for developing vaccines. © 1990, Mary Ann Liebert, Inc. All rights reserved.
引用
收藏
页码：813 / 823
页数：11
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