PHYLOGENETIC STUDY ON VERTEBRATE MITOCHONDRIAL-DNA POLYMERASE

We have started a phylogenetic survey for the mitochondrial DNA polymerase and present in this study the results obtained for all the different classes for the vertebrates. The operating conditions include the purification of mitochondria, the analysis of the DNA polymerase activity in the extract and the determination of the sedimentation coefficient on sucrose gradients. The utilization of digitonin for removing the external membrane of the organelle and contaminating proteins has been generalized since this detergent shows no effect on the activities of either DNA polymerase α or γ. The results obtained for the mitochondria of different classes of vertebrates show that the activity responding to the specific assay of DNA polymerase γ tended invariably to increase during purification while that of DNA polymerase α tended to decrease. Furthermore in almost all the cases the γ‐polymerase represented the only DNA polymerase activity found in the mitochondria after digitonin treatment. The analysis of the sedimentation patterns of the mitochondrial DNA polymerase strongly suggests the presence of a single type of DNA polymerase showing the typical properties of the γ‐polymerase. It is concluded that the vertebrate mitochondria contain a well‐defined and unique form of DNA polymerase which corresponds to the DNA polymerase γ. Copyright © 1979, Wiley Blackwell. All rights reserved