EPINEPHRINE AND GLUCAGON COUNTERACT INHIBITION OF PROTEIN-SYNTHESIS INDUCED BY D-GALACTOSAMINE IN ISOLATED MOUSE HEPATOCYTES

10 mM d-galactosamine inhibited protein synthesis (1 h incubation time) by 67% in isolated mouse liver cells. Counteracting uridylate deficiency induced by d-galactosamine by preventive administration of 20 mM uridine did not decrease the extent of protein synthesis inhibition. 20 mM d-galactose reverted the inhibition of protein synthesis by d-galactosamine. 10-5M epinephrine and 10-7 M glucagon decreased the incorporation of d-galactosamine into glycogen to 38% and 26% of the control value, respectively, after a 35 min incubation and reduced the inhibition of protein synthesis by d-glactosamine effectively. Experimental evidence supports the view that aminoglycogen formed after d-galactosamine treatment is responsible for the inhibition of protein synthesis. © 1979.