ROLE OF 5S RIBOSOMAL RNA IN POLYPEPTIDE SYNTHESIS

被引：11
作者：
SIDDIQUI, MA
HOSOKAWA, K
机构：
[1] Space Sciences Laboratory, University of California, Berkeley
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1969年 / 36卷 / 05期
基金：
美国国家航空航天局; 美国国家卫生研究院;
关键词：
D O I：
10.1016/0006-291X(69)90668-8
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Complete dissociation of 5S rRNA was obtained by mild treatment of 50S ribosomal subunits with EDTA. The 5S rRNA-free 50S subunits (50S E) could bind 30S subunits. The complex became attached to phenylalanyl-tRNA in the presence of poly U. The tRNA binding, however, was only 50% of that observed with normal ribosomes, and was resistant to tetracycline inhibition. Phenylalanyl-tRNA bound to 5S-rRNA-free ribosome-poly U complex was sensitive to pancreatic RNase. These results suggest that 5S rRNA may have a role in the specific binding of tRNA to ribosomes. © 1969.
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页码：711 / &
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