SYNTHESIS OF A 5'-THIOPHOSPHATE ANALOG OF 2-5A, A PHOSPHATASE RESISTANT ACTIVATOR OF THE 2-5A-DEPENDENT RIBONUCLEASE

被引：27
作者：
XIAO, W
LI, GY
LESIAK, K
DONG, BH
SILVERMAN, RH
TORRENCE, PF
机构：
[1] NIDDK,MED CHEM LAB,BIOMED CHEM SECT,BETHESDA,MD 20892
[2] US FDA,CTR BIOL EVALUAT & RES,DIV ALLERGEN PROD & PARASITOL,BIOPHYS LAB,BETHESDA,MD 20892
[3] CLEVELAND CLIN FDN,RES INST,DEPT CANC BIOL,CLEVELAND,OH 44195
来源：
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS | 1994年 / 4卷 / 21期
关键词：
D O I：
10.1016/S0960-894X(01)80294-8
中图分类号：
R914 [药物化学];
学科分类号：
100701 ;
摘要：
The 5'-thiophosphate analogue of the small oligonucleotide mediator of the anti-picornavirus action of interferon was synthesized and shown to be highly resistant to enzymic 5'-dephosphorylation and to activate the 2-5A-dependent ribonuclease as effectively as parent p5'A2'p5'A2'p5'A itself.
引用
收藏
页码：2609 / 2614
页数：6
相关论文
共 14 条
[1]  
DONG BH, 1994, J BIOL CHEM, V269, P14153
[2]   INVESTIGATION OF ENZYME MECHANISMS WITH NUCLEOSIDE PHOSPHOROTHIOATES [J].
ECKSTEIN, F .
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 1975, 14 (03) :160-166
[3]   PHOSPHOROTHIOATE ANALOGS OF NUCLEOTIDES - TOOLS FOR THE INVESTIGATION OF BIOCHEMICAL PROCESSES [J].
ECKSTEIN, F .
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 1983, 22 (06) :423-439
[4]   PHOSPHOROTHIOATE ANALOGS OF NUCLEOTIDES [J].
ECKSTEIN, F .
ACCOUNTS OF CHEMICAL RESEARCH, 1979, 12 (06) :204-210
[5]   A CHEMICAL 5'-PHOSPHORYLATION OF OLIGODEOXYRIBONUCLEOTIDES THAT CAN BE MONITORED BY TRITYL CATION RELEASE [J].
HORN, T ;
URDEA, MS .
TETRAHEDRON LETTERS, 1986, 27 (39) :4705-4708
[6]   3H-1,2-BENZODITHIOLE-3-ONE 1,1-DIOXIDE AS AN IMPROVED SULFURIZING REAGENT IN THE SOLID-PHASE SYNTHESIS OF OLIGODEOXYRIBONUCLEOSIDE PHOSPHOROTHIOATES [J].
IYER, RP ;
EGAN, W ;
REGAN, JB ;
BEAUCAGE, SL .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1990, 112 (03) :1253-1254
[7]  
JOHNSTON ML, 1984, INTERFERON MECHANISM, P189
[8]   2',5'-OLIGOADENYLATE - ANTISENSE CHIMERAS SYNTHESIS AND PROPERTIES [J].
LESIAK, K ;
KHAMNEI, S ;
TORRENCE, PF .
BIOCONJUGATE CHEMISTRY, 1993, 4 (06) :467-472
[9]   FUNCTIONAL-ANALYSIS OF 2-5A-DEPENDENT RNASE AND 2-5A USING 2',5'-OLIGOADENYLATE-CELLULOSE [J].
SILVERMAN, RH .
ANALYTICAL BIOCHEMISTRY, 1985, 144 (02) :450-460
[10]   CHEMICAL SYNTHESIS AND BIOLOGICAL CHARACTERIZATION OF PHOSPHOROTHIOATE ANALOGS OF 2',5'-3'-DEOXYADENYLATE TRIMER [J].
SOBOL, RW ;
CHARUBALA, R ;
PFLEIDERER, W ;
SUHADOLNIK, RJ .
NUCLEIC ACIDS RESEARCH, 1993, 21 (10) :2437-2443
12