FORMATION AND PROPERTIES OF A STABLE HIGH-POTENTIAL COPPER-IRON-SULFUR CLUSTER IN A FERREDOXIN

被引：33
作者：
BUTT, JN
NILES, J
ARMSTRONG, FA
BRETON, J
THOMSON, AJ
机构：
[1] UNIV OXFORD,INORGAN CHEM LAB,OXFORD OX1 3QR,ENGLAND
[2] UNIV E ANGLIA,SCH CHEM SCI,NORWICH NR4 7TJ,NORFOLK,ENGLAND
来源：
NATURE STRUCTURAL BIOLOGY | 1994年 / 1卷 / 07期
关键词：
D O I：
10.1038/nsb0794-427
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A ferredoxin isolated from Desulfovibrio africanus contains a [3Fe-4S] cluster that reversibly binds a copper atom, yielding a stable product with a greatly increased reduction potential. The reaction is readily detected in protein molecules adsorbed as a film on an electrode surface. Electron paramagnetic resonance (EPR) and magnetic circular dichroism (MCD) spectra of oxidized and reduced bulk solution products support their assignment as [Cu3Fe-4S](2+) (S=1/2) and [Cu3Fe4S](1+) (S=2) respectively with copper bound formally as Cu(I). Cyanide causes selective loss of copper and regeneration of the [3Fe-4S] reactant. The results demonstrate the chemical feasibility of CuFeS clusters and suggest that they could exist naturally in biological systems.
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页码：427 / 433
页数：7
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