SIMULTANEOUS IDENTIFICATION OF STRAINS OF ESCHERICHIA-COLI SEROTYPE O157-H7 AND THEIR SHIGA-LIKE TOXIN TYPE BY MISMATCH AMPLIFICATION MUTATION ASSAY-MULTIPLEX PCR

被引：341

作者：

CEBULA, TA ^{[1
]}

PAYNE, WL ^{[1
]}

FENG, P ^{[1
]}

机构：

[1] US FDA,CTR FOOD SAFETY & APPL NUTR,WASHINGTON,DC 20204

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 1995年 / 33卷 / 01期

关键词：

D O I：

10.1128/JCM.33.1.248-250.1995

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Mismatch amplification mutation assay primers, specific for a unique base substitution in uidA of Escherichia call O157:H7, was coupled with primers for the Shiga-like toxin I (SLT-I) and SLT-II genes in a multiplex PCR assay. Analysis of 108 bacteria showed that all Escherichia coli serotype O157:H7 strains were identified simultaneously with the SLT types encoded by these strains.

引用

页码：248 / 250

页数：3

共 18 条

[1] ENZYME-LINKED IMMUNOSORBENT ASSAYS FOR DETECTING ANTIBODIES TO SHIGA-LIKE TOXIN-I, SHIGA-LIKE TOXIN-II, AND ESCHERICHIA-COLI O157-H7 LIPOPOLYSACCHARIDE IN HUMAN SERUM [J].

BARRETT, TJ ;

GREEN, JH ;

GRIFFIN, PM ;

PAVIA, AT ;

OSTROFF, SM ;

WACHSMUTH, IK .

CURRENT MICROBIOLOGY, 1991, 23 (04) :189-195

[2] ISOLATION OF A CITROBACTER-FREUNDII STRAIN WHICH CARRIES THE ESCHERICHIA-COLI O157 ANTIGEN [J].

BETTELHEIM, KA ;

EVANGELIDIS, H ;

PEARCE, JL ;

SOWERS, E ;

STROCKBINE, NA .

JOURNAL OF CLINICAL MICROBIOLOGY, 1993, 31 (03) :760-761

[3]

BOKETE TN, 1993, GASTROENTEROLOGY, V105, P1724, DOI 10.1016/0016-5085(93)91069-T

[4]

Cha R S, 1992, PCR Methods Appl, V2, P14

[5] IDENTIFICATION OF UIDA GENE-SEQUENCES IN BETA-D-GLUCURONIDASE-NEGATIVE ESCHERICHIA-COLI [J].

FENG, P ;

LUM, R ;

CHANG, GW .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1991, 57 (01) :320-323

[6] IDENTIFICATION OF ESCHERICHIA-COLI SEROTYPE O157-H7 BY DNA PROBE SPECIFIC FOR AN ALLELE OF UID A-GENE [J].

FENG, P .

MOLECULAR AND CELLULAR PROBES, 1993, 7 (02) :151-154

[7] GENETIC-ANALYSIS OF UIDA EXPRESSION IN ENTEROHEMORRHAGIC ESCHERICHIA-COLI SEROTYPE O157-H7 [J].

FENG, P ;

LAMPEL, KA .

MICROBIOLOGY-SGM, 1994, 140 :2101-2107

[8] VIRULENCE OF AN ESCHERICHIA-COLI O157-H7 SORBITOL-POSITIVE MUTANT [J].

FRATAMICO, PM ;

BUCHANAN, RL ;

COOKE, PH .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1993, 59 (12) :4245-4252

[9] THE EPIDEMIOLOGY OF INFECTIONS CAUSED BY ESCHERICHIA-COLI O157-H7, OTHER ENTEROHEMORRHAGIC ESCHERICHIA-COLI, AND THE ASSOCIATED HEMOLYTIC UREMIC SYNDROME [J].

GRIFFIN, PM ;

TAUXE, RV .

EPIDEMIOLOGIC REVIEWS, 1991, 13 :60-98

[10] MOLECULAR-DETECTION OF SORBITOL-FERMENTING ESCHERICHIA-COLI O157 IN PATIENTS WITH HEMOLYTIC-UREMIC SYNDROME [J].

GUNZER, F ;

BOHM, H ;

RUSSMANN, H ;

BITZAN, M ;

ALEKSIC, S ;

KARCH, H .

JOURNAL OF CLINICAL MICROBIOLOGY, 1992, 30 (07) :1807-1810

← 1 2 →