THE ROLE OF CELLULAR COMPETITION IN B-CELL SURVIVAL AND SELECTION OF B-CELL REPERTOIRES

被引:60
作者
FREITAS, AA [1 ]
ROSADO, MM [1 ]
VIALE, AC [1 ]
GRANDIEN, A [1 ]
机构
[1] INST PASTEUR, CNRS, URA 1961, UNITE IMMUNOBIOL, F-75015 PARIS, FRANCE
关键词
B LYMPHOCYTE; REPERTOIRE SELECTION; COMPETITION;
D O I
10.1002/eji.1830250636
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
We studied the competitive repopulation by different B cells of irradiated mice reconstituted with bone marrow from either congenic or Ig-transgenic (TG) mice mixed at different ratios. We found that after reconstitution, the number of B cells recovered in the different chimeras is similar and independent of the ratio of injected cells. In chimeras hosting TG and non-TG cells, the relative representation of the donor cell lineages diverges from the ratios present in the inoculum, i.e. at the periphery, non-TG cells are preferentially selected. Selection of non-TG cells only occurs when population growth plateaus, i.e. when resources become limiting and competition starts to operate. Selection of non-TG cells depends on surface Ig expression, and they are selected because they have a longer survival. Finally, the life-expectancy of the same B cell population differs depending upon the second population present. The present results show that the life-span and the population size of each B cell clone can be altered (interfered with) by the presence of a second cell population, demonstrating the existence of cellular competition among B cells. Our findings establish the role of cellular competition in the selection of B cell repertoires and the existence of a hierarchy of B cell selection in the absence of antigenic stimulation. The implications of cellular competition on our understanding of the immune system are discussed.
引用
收藏
页码:1729 / 1738
页数:10
相关论文
共 49 条
[1]  
Aherne W. A., 1977, INTRO CELL POPULATIO
[2]  
BELL EB, 1975, EUR J IMMUNOL, V5, P1
[3]   MIGRATION OF BONE MARROW LYMPHOCYTES DEMONSTRATED BY SELECTIVE BONE MARROW LABELING WITH THYMIDINE H-3 [J].
BRAHIM, F ;
OSMOND, DG .
ANATOMICAL RECORD, 1970, 168 (02) :139-&
[4]   DIFFERENTIAL REGULATION OF EARLY AND LATE STAGES OF B-LYMPHOCYTE DEVELOPMENT BY THE MU-MEMBRANE AND DELTA-MEMBRANE HEAVY-CHAINS OF IG [J].
BRINK, R ;
FULCHER, DA ;
GOODNOW, CC ;
BASTEN, A .
INTERNATIONAL IMMUNOLOGY, 1994, 6 (12) :1905-1916
[5]  
Buss LW., 1987, EVOLUTION INDIVIDUAL
[6]   A MONOCLONAL-ANTIBODY THAT RECOGNIZES B-CELLS AND B-CELL PRECURSORS IN MICE [J].
COFFMAN, RL ;
WEISSMAN, IL .
JOURNAL OF EXPERIMENTAL MEDICINE, 1981, 153 (02) :269-279
[7]   COMPETITION FOR FOLLICULAR NICHES EXCLUDES SELF-REACTIVE CELLS FROM THE RECIRCULATING B-CELL REPERTOIRE [J].
CYSTER, JG ;
HARTLEY, SB ;
GOODNOW, CC .
NATURE, 1994, 371 (6496) :389-395
[8]   HOW DIVERSE SHOULD THE IMMUNE-SYSTEM BE [J].
DEBOER, RJ ;
PERELSON, AS .
PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES, 1993, 252 (1335) :171-175
[9]  
Freitas A A, 1989, Int Immunol, V1, P342, DOI 10.1093/intimm/1.4.342
[10]   SELECTION OF VH GENE REPERTOIRES - DIFFERENTIATING B-CELLS OF ADULT BONE-MARROW MIMIC FETAL DEVELOPMENT [J].
FREITAS, AA ;
ANDRADE, L ;
LEMBEZAT, MP ;
COUTINHO, A .
INTERNATIONAL IMMUNOLOGY, 1990, 2 (01) :15-23