DETERMINATION OF C-13 ENRICHMENT BY CONVENTIONAL GC-MS AND GC-(MS)-C-IRMS

被引：6

作者：

Meier-Augenstein, W. ^{[1
]}

Rating, D. ^{[2
]}

Hoffmann, G. F. ^{[2
]}

Wendel, U. ^{[3
]}

Matthiesen, U. ^{[5
]}

Schadewaldt, P. ^{[4
]}

机构：

[1] Univ Dundee, Dept Anat & Physiol, Dundee DD1 4HN, Scotland

[2] Univ Heidelberg, Kinderklin, D-6900 Heidelberg, Germany

[3] Univ Nijmegen, Akad Ziekenhuis, Nijmegen, Netherlands

[4] Univ Dusseldorf, Diabet Forschungsinst, Dusseldorf, Germany

[5] Univ Dusseldorf, Spurenelementlab, Dusseldorf, Germany

来源：

ISOTOPES IN ENVIRONMENTAL AND HEALTH STUDIES | 1995年 / 31卷 / 3-4期

关键词：

branched-chain amino acids; GC-C-IRMS; GC-MS; MSUD; stable isotope analysis; selected ion monitoring; tracer techniques;

D O I：

10.1080/10256019508036268

中图分类号：

O61 [无机化学];

学科分类号：

070301 ; 081704 ;

摘要：

Isotopic enrichment of branched-chain L-amino acids ( BCAA) and branched-chain 2-oxo acids ( BCOA) in standard preparations and in human plasma samples withdrawn after oral loads with 1-C-13 labelled BCAA were measured on a conventional GC-MS system and an on-line GC-C-IRMS employing O-TMS quinoxalinol derivatives. It was concluded that the recently introduced GC-C-IRMS, owing to its high sensitivity, is the adequate analytical tool when tracer doses of stable isotope labelled compounds of low enrichment are to be used in biomedical in vivo studies.

引用

页码：261 / 266

页数：6

共 5 条

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[3] DETERMINATION OF ISOTOPE ENRICHMENT IN C-13-LABELED OR H-2-LABELED BRANCHED-CHAIN L-AMINO-ACIDS FROM PHYSIOLOGICAL FLUIDS BY GAS-CHROMATOGRAPHY MASS-SPECTROMETRY - USE OF L-LEUCINE DEHYDROGENASE FOR SPECIFIC PREPARATION OF THE QUINOXALINOL DERIVATIVES
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[J]. ISOTOPENPRAXIS, 1994, 30 (2-3): : 219 - 230
[4] BRIDGING THE INFORMATION GAP BETWEEN ISOTOPE RATIO MASS-SPECTROMETRY AND CONVENTIONAL MASS-SPECTROMETRY
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BRAND, W
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[5] LEUCINE OXIDATION IN-VIVO - INTERINDIVIDUAL AND INTRAINDIVIDUAL VARIATION IN HEALTHY-SUBJECTS AS ASSESSED BY ORAL L-[1-C-13]LEUCINE LOADS
SCHADEWALDT, P
BROSICKE, H
HAISCH, M
MATTHIESEN, U
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[J]. ISOTOPENPRAXIS, 1994, 30 (2-3): : 141 - 150

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