INHIBITION OF MICROTUBULE ASSEMBLY INVITRO BY ANTITUBULIN MONOCLONAL-ANTIBODIES

被引：5

作者：

DRABER, P ^{[1
]}

VATER, W ^{[1
]}

BOHM, KJ ^{[1
]}

KUKLOVA, E ^{[1
]}

UNGER, E ^{[1
]}

机构：

[1] ACAD SCI GDR, CENT INST MICROBIOL & EXPTL THERAPY, O-6900 JENA, GERMANY

来源：

FEBS LETTERS | 1990年 / 262卷 / 02期

关键词：

Microtubule assembly; Microtubule protein; Monoclonal antibody; Tubulin domain;

D O I：

10.1016/0014-5793(90)80191-K

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Five monoclonal antibodies against N-terminal domains of α- or β-tubulin were tested for their ability to interfere with the in vitro formation of microtubules. Although all the antibodies exhibited similar association constants for immobilized tubulin, they differed in their inhibitory effect on microtubule assembly. For the most potent antibody, TU-13, the antibody/tubulin molar ratio of about 1:320 was sufficient for a 50% inhibition. The data indicate that the surface regions of N-terminal domains of tubulin are involved in the formation of microtubules. © 1990.

引用

页码：209 / 211

页数：3

共 18 条

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