RAPID ALKALINE REHYDRATION OF DRIED PLANT TISSUES FOR HISTOLOGIC STUDY

被引：9

作者：

CUNNINGHAM, JL

机构：

[1] Mycology Investigations, Crops Research Division, Agricultural Research Service, U.S. Department of Agriculture, Beltsville, MD

来源：

STAIN TECHNOLOGY | 1969年 / 44卷 / 05期

关键词：

D O I：

10.3109/10520296909063359

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Pieces of leaf tissue were soaked in 3% aqueous KOH at about 75 C for 20-30 min, washed, evacuated, dehydrated, embedded in paraffin, and 5-7 μ sections attached to slides with gelatin adhesive. Compared with fresh-fixed control material, dried material stained with less contrast, but the general morphology of cells and tissues was fully restored. Nuclei and chloroplasts were preserved only occasionally, but such delicate structures as hyphae, haustoria, and parenchyma were regularly restored to normally appearing turgor. Long-term storage of dried specimens (one for 84 yr; another 100 yr) was not at all detrimental to restoration. © 1969 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted.

引用

页码：243 / +

页数：1