NONISOTOPIC IN-SITU DETECTION OF MESSENGER-RNA FOR INTERLEUKIN-4 IN ARCHIVAL HUMAN TISSUE

被引：12
|
作者：
BROMLEY, L [1 ]
MCCARTHY, SP [1 ]
STICKLAND, JE [1 ]
LEWIS, CE [1 ]
MCGEE, JOD [1 ]
机构：
[1] UNIV OXFORD,JOHN RADCLIFFE HOSP,NUFFIELD DEPT PATHOL & BACTERIOL,OXFORD OX3 9DU,ENGLAND
来源：
JOURNAL OF IMMUNOLOGICAL METHODS | 1994年 / 167卷 / 1-2期
关键词：
MESSENGER-RNA; IL-4; NONISOTOPIC IN SITU HYBRIDIZATION; ARCHIVAL MATERIAL;
D O I：
10.1016/0022-1759(94)90073-6
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Non-isotopic in situ hybridisation (NISH) is a rapid and sensitive method currently used in molecular pathology to identify DNA in fresh and archival human biopsies. In this study we have extended NISH technology for mRNA detection to include the use of digoxigenin-labelled riboprobes to localise low abundance mRNA for interleukin-4 in paraffin embedded material (from active inflammatory bowel disease). As this methodological approach makes the retrospective study of cytokine gene expression in archival material possible for the first time, it could act as a prototype for studies of cytokine involvement in various human diseases.
引用
收藏
页码：47 / 54
页数：8
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