LECTIN GENES FROM THE LEGUME MEDICAGO-TRUNCATULA

被引：19

作者：

BAUCHROWITZ, MA ^{[1
]}

BARKER, DG ^{[1
]}

NADAUD, I ^{[1
]}

ROUGE, P ^{[1
]}

LESCURE, B ^{[1
]}

机构：

[1] FAC SCI PHARMACEUT TOULOUSE, BIOL CELLULAIRE LAB, F-31062 TOULOUSE, FRANCE

来源：

PLANT MOLECULAR BIOLOGY | 1992年 / 19卷 / 06期

关键词：

FRAMESHIFT MUTATION; LECTIN GENES; MEDICAGO-TRUNCATULA;

D O I：

10.1007/BF00040532

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We report the cloning and characterization of two lectin genes from Medicago truncatula, designated Mtlec1 and Mtlec2. The two genes show a high degree of homolog and apparently belong to a small multigene family. Mtlec1 appears to encode a functional lectin with 277 amino acids, whereas Mtlec2 is probably non-functional, since a frameshift mutation (insertion of two nucleotides) leads to premature translation termination after only 98 amino acids. The deduced amino acid sequence of the polypeptide MtLEC1 suggests that this lectin is a metalloprotein with Glc/Man specificity.

引用

页码：1011 / 1017

页数：7

共 30 条

[1]

Barker D. G., 1990, Plant Molecular Biology Reporter, V8, P40, DOI 10.1007/BF02668879

[2] IDENTIFICATION OF 2 GROUPS OF LEGHEMOGLOBIN GENES IN ALFALFA (MEDICAGO-SATIVA) AND A STUDY OF THEIR EXPRESSION DURING ROOT NODULE DEVELOPMENT [J].

BARKER, DG ;

GALLUSCI, P ;

LULLIEN, V ;

KHAN, H ;

GHERARDI, M ;

HUGUET, T .

PLANT MOLECULAR BIOLOGY, 1988, 11 (06) :761-772

[3] LECTINS - POSSIBLE BASIS FOR SPECIFICITY IN RHIZOBIUM-LEGUME ROOT NODULE SYMBIOSIS [J].

BOHLOOL, BB ;

SCHMIDT, EL .

SCIENCE, 1974, 185 (4147) :269-271

[4] 3-DIMENSIONAL STRUCTURES OF COMPLEXES OF LATHYRUS-OCHRUS ISOLECTIN-I WITH GLUCOSE AND MANNOSE - FINE SPECIFICITY OF THE MONOSACCHARIDE-BINDING SITE [J].

BOURNE, Y ;

ROUSSEL, A ;

FREY, M ;

ROUGE, P ;

FONTECILLACAMPS, JC ;

CAMBILLAU, C .

PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS, 1990, 8 (04) :365-376

[5] X-RAY CRYSTAL-STRUCTURE DETERMINATION AND REFINEMENT AT 1.9 A RESOLUTION OF ISOLECTIN-I FROM THE SEEDS OF LATHYRUS-OCHRUS [J].

BOURNE, Y ;

ABERGEL, C ;

CAMBILLAU, C ;

FREY, M ;

ROUGE, P ;

FONTECILLACAMPS, JC .

JOURNAL OF MOLECULAR BIOLOGY, 1990, 214 (02) :571-584

[6] FAVIN VERSUS CONCANAVALIN-A - CIRCULARLY PERMUTED AMINO-ACID SEQUENCES [J].

CUNNINGHAM, BA ;

HEMPERLY, JJ ;

HOPP, TP ;

EDELMAN, GM .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1979, 76 (07) :3218-3222

[7] ROOT LECTIN AS A DETERMINANT OF HOST-PLANT SPECIFICITY IN THE RHIZOBIUM-LEGUME SYMBIOSIS [J].

DIAZ, CL ;

MELCHERS, LS ;

HOOYKAAS, PJJ ;

LUGTENBERG, BJJ ;

KIJNE, JW .

NATURE, 1989, 338 (6216) :579-581

[8]

EINSPAHR H, 1986, J BIOL CHEM, V261, P6518

[9] PLANT-LECTINS - MOLECULAR AND BIOLOGICAL ASPECTS [J].

ETZLER, ME .

ANNUAL REVIEW OF PLANT PHYSIOLOGY AND PLANT MOLECULAR BIOLOGY, 1985, 36 :209-234

[10] A TECHNIQUE FOR RADIOLABELING DNA RESTRICTION ENDONUCLEASE FRAGMENTS TO HIGH SPECIFIC ACTIVITY [J].

FEINBERG, AP ;

VOGELSTEIN, B .

ANALYTICAL BIOCHEMISTRY, 1983, 132 (01) :6-13

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