MEASUREMENT OF TESTOSTERONE WITH A HIGH-PERFORMANCE LIQUID CHROMATOGRAPH EQUIPPED WITH A FLOW-THROUGH ULTRAVIOLET SPECTROPHOTOMETER

被引：29
作者：
COCHRAN, RC
DARNEY, KJ
EWING, LL
机构：
[1] Division of Reproductive Biology, Department of Population Dynamics, The Johns Hopkins University School of Hygiene and Public Health, Baltimore, MD 21205
来源：
JOURNAL OF CHROMATOGRAPHY | 1979年 / 173卷 / 02期
关键词：
D O I：
10.1016/S0021-9673(00)92303-3
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A technique is described for measuring nanogram amounts of testosterone using high-performance liquid chromatography with detection by a flow-through spectrophotometer. The addition of a non-radioisotopic internal standard (4-androsten-11β-ol-3,17-dione) to the biological specimen automatically corrects for testosterone losses due to extraction and non-quantitative sample injection into the high-performance liquid chromatograph. This new method, which can be performed by inexperienced personnel, is shown to be rapid, precise, accurate and specific for testosterone. © 1979.
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页码：349 / 355
页数：7
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