MEASUREMENT OF TESTOSTERONE WITH A HIGH-PERFORMANCE LIQUID CHROMATOGRAPH EQUIPPED WITH A FLOW-THROUGH ULTRAVIOLET SPECTROPHOTOMETER

被引：29

作者：

COCHRAN, RC

DARNEY, KJ

EWING, LL

机构：

[1] Division of Reproductive Biology, Department of Population Dynamics, The Johns Hopkins University School of Hygiene and Public Health, Baltimore, MD 21205

来源：

JOURNAL OF CHROMATOGRAPHY | 1979年 / 173卷 / 02期

关键词：

D O I：

10.1016/S0021-9673(00)92303-3

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A technique is described for measuring nanogram amounts of testosterone using high-performance liquid chromatography with detection by a flow-through spectrophotometer. The addition of a non-radioisotopic internal standard (4-androsten-11β-ol-3,17-dione) to the biological specimen automatically corrects for testosterone losses due to extraction and non-quantitative sample injection into the high-performance liquid chromatograph. This new method, which can be performed by inexperienced personnel, is shown to be rapid, precise, accurate and specific for testosterone. © 1979.

引用

页码：349 / 355

页数：7

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