EXPERIMENTS, CORRELATION AND PREDICTION OF PROTEIN PARTITION-COEFFICIENT IN AQUEOUS 2-PHASE SYSTEMS CONTAINING PEG AND K2HPO4-KH2PO4

被引：30

作者：

PENG, QH ^{[1
]}

LI, ZC ^{[1
]}

LI, YG ^{[1
]}

机构：

[1] TSING HUA UNIV,DEPT CHEM ENGN,BEIJING 100084,PEOPLES R CHINA

来源：

FLUID PHASE EQUILIBRIA | 1995年 / 107卷 / 02期

基金：

中国国家自然科学基金;

关键词：

EXPERIMENTS; DATA; METHOD; LIQUID-LIQUID EQUILIBRIA; LATTICE MODEL; PARTITION COEFFICIENT; ELECTROLYTE; POLYMER; PROTEIN;

D O I：

10.1016/0378-3812(94)02674-P

中图分类号：

O414.1 [热力学];

学科分类号：

摘要：

A quantitative analytical method for the study of the liquid-liquid equilibria (LLE) of K2HPO4-KH2PO4-PEG-protein aqueous two-phases systems has been established. 23 partition coefficients for six proteins at 25 degrees C were measured. The Baskir adsorption lattice model and Fitter long-range electrostatic term were extended to calculate the protein partition coefficients in aqueous two-phase systems containing electrolyte and polymer. Two adsorption energy parameters were regressed from six sets of experimental data of lysozyme-containing systems and can predict the other 17 partition coefficients of lysozyme and five other proteins in aqueous two-phase systems. The average relative deviation of the partition coefficient between experiment and calculation is less than 6%. The factors affecting protein partition coefficient were discussed.

引用

页码：303 / 315

页数：13

共 50 条

[21] Measurement and correlation of density for PEG+H2O+NaHSO4, NaH2PO4, and Na2HPO4 at three temperatures
Zafarani-Moattar, MT
Mehrdad, A
JOURNAL OF CHEMICAL AND ENGINEERING DATA, 2000, 45 (02): : 386 - 390
[22] Nitrogen loss reduction by adding KH2PO4-K2HPO4 buffer solution during composting of sewage sludge
Liang, Jiayun
Shen, Yanwen
Shou, Zongqi
Yuan, Haiping
Dai, Xiaohu
Zhu, Nanwen
BIORESOURCE TECHNOLOGY, 2018, 264 : 116 - 122
[23] THE EFFECT OF HYDROCHLORIC-ACID ON HYDROPHOBICITY AND PARTITION OF PROTEIN IN AQUEOUS 2-PHASE SYSTEMS
TANAKA, H
KUBOI, R
KOMASAWA, I
JOURNAL OF CHEMICAL ENGINEERING OF JAPAN, 1991, 24 (05) : 661 - 664
[24] Phase Equilibria in the KNO3-KH2PO4-K2SO4-H2O System at 298.2 K
Kistanova, Natalya
Shabanov, Roman
Korotkikh, Sergei
Kudryashova, Olga
JOURNAL OF CHEMICAL AND ENGINEERING DATA, 2024, 69 (09): : 3108 - 3115
[25] Phase Equilibrium for the Ternary System KH2PO4 + NaH2PO4 + H2O at 303.15 K
Shen, Si
Tang, Jian-hua
Liu, Zhong-hua
Kang, Li-quan
Mao, Dun
JOURNAL OF CHEMICAL AND ENGINEERING DATA, 2015, 60 (04): : 1072 - 1075
[26] Aqueous solutions of NMA, Na2HPO4, and NaH2PO4 as models for interaction studies in phosphate-protein systems
Biernacki, Karol A.
Kaczkowska, Emilia
Bruzdziak, Piotr
JOURNAL OF MOLECULAR LIQUIDS, 2018, 265 : 361 - 371
[27] Liquid-liquid equilibrium data for aqueous solutions of surfactant Brij 58 with (NH4)H2PO4, (NH4)2HPO4, and KH2PO4
Foroutan, Masumeh
Heidari, Nosrat
Mohammadlou, Maryam
JOURNAL OF CHEMICAL AND ENGINEERING DATA, 2008, 53 (01): : 242 - 246
[28] NMR OF K39 IN KH2PO4 IN FERROELECTRIC PHASE
TSUTSUMI, Y
KUNITOMO, M
TERAO, T
HASHI, T
JOURNAL OF THE PHYSICAL SOCIETY OF JAPAN, 1968, 24 (02) : 429 - &
[29] Study of phase equilibria in the CO(NH2)2-KH2PO4-K2HPO4-H2O system at 25°C using an optimized method of cross sections
M. N. Noskov
S. A. Mazunin
Russian Journal of Physical Chemistry A, 2015, 89 : 1011 - 1017
[30] Partitioning of some amino acids and low molecular mass peptides in aqueous two-phase systems of poly(ethylene glycol) and dextran in the presence of small amounts of K2HPO4/KH2PO4-buffer at 293 K: Experimental results and correlation
Tintinger, R
Zhu, JD
Grossmann, C
Maurer, G
JOURNAL OF CHEMICAL AND ENGINEERING DATA, 1997, 42 (05): : 975 - 984

← 1 2 3 4 5 →