PROTOPLAST FUSION OF BETA-GLUCOSIDASE-PRODUCING ASPERGILLUS-NIGER STRAINS

被引：6
作者：
HOH, YK
TAN, TK
YEOH, HH
机构：
[1] Department of Botany, Faculty of Science, National University of Singapore, Singapore, 0511
来源：
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY | 1992年 / 37卷 / 01期
关键词：
BETA-GLUCOSIDASE; CELLOBIOSE HYDROLYSIS; PROTOPLAST FUSION; ASPERGILLUS-NIGER;
D O I：
10.1007/BF02788859
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Protoplast fusion, induced by polyethylene glycol and Ca2+, was carried out between two auxotrophic strains of Aspergillus niger. The fusion frequency ranged from 6.2 x 10(-2)-9.1 X 10(-2). After induced haploidization of a diploid, various segregants showing combinations of the parental genetic markers were isolated. Unlike diploids, haploid segregants exhibited greater variations in their morphology and beta-glucosidase activities. One segregant showed a 2.5-fold increase in beta-glucosidase activity over those of the parents. Thus, this method appears promising for creating new recombinant strains of A. niger with improved beta-glucosidase activities.
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页码：81 / 88
页数：8
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